隨著人口結構老年化，慢性病的罹患機率增加，使得慢性傷口的問題也越來越複雜，由於慢性傷口癒合不易，若處理不妥將對患者造成傷害且加重醫療資源與人員的消耗與負擔。為促進傷口的細胞增生效率，光治療法已經被廣泛應用於臨床醫學上，因此本論文將提出一低能量光治療法之治療系統，針對傷口狀態以相呼應波長之光能量進行照射治療，同時所提出之基於恆流源LED陣列之區域調光演算法，不僅能提升傷口照射面之光均勻度，更可對特定區域進行指定輻照度的光治療，提升慢性傷口癒合能力。
本系統採用415 nm藍光來進行殺菌，並以633 nm紅光和850 nm紅外光來促進增生期的成纖維母細胞活化，藉由不同輻照度及照射時間來進行光生物調解之體外細胞實驗。而為避免LED陣列之光疊加效應所導致的光均勻度不均問題，本系統針對LED之不同工作週期進行輻照度量測，並透過所建立之光源衰減範圍矩陣，建構出基於最小平方 (least square)之區域調光演算法。由實驗結果得知，本系統對L929細胞之光照實驗之最佳參數為633 nm紅光操作在輻照度15 mW/cm2下給予3 J/cm2能量密度，可讓L929有最佳細胞增生率。而在殺菌實驗中則是以藍光操作在25 mW/cm2、50 J/cm2時可對綠膿桿菌產生顯著的滅菌效果。此外，本論文亦透過調光演算法以MATLAB模擬得到之理想輻照度值與實際量測值可計算出在理想輻照度5 mW/cm2、15 mW/cm2、25 mW/cm2下平均誤差分別為1.93%、1.73%、1.4%，顯示本系統在輻照度的穩定性與強健性，且由光學量測結果顯示陣列均勻度最高可達97.9%，亦說明本系統具有高均勻度的特性進而降低細胞實驗的變異性。最後本論文也展示初步傷口區域影像辨識結果，藉由導入RCNN模型來選取傷口區域面積，以增強治療系統對傷口修復之泛用性。

This work presents a Low-Level Light Therapy (LLLT) system which can irradiate in a specific wavelength of light and energy to the corresponding state of a wound in order to promote tissue healing. Moreover, this work proposes a LED-based local dimming algorithm which can optimize the uniformity of the irradiated position, helping improve wound healing. This system utilizes a blue light of 415 nm to sterilize Pseudomonas aeruginosa and adopts a red light of 633 nm and infrared of 850 nm to enhance the activity of fibroblast. An in vitro experiment of photobiomodulation on L929 cell cultures was conducted over several irradiance and time periods to evaluate the feasibility of the proposed system in healing wounds. Furthermore, to avoid the experimental variation caused by the light superposition principle, the local dimming algorithm is proposed based on the least square method to adjust the irradiance of each LED. The experimental results demonstrate that the red light (633nm) with an irradiance of 15 mW/cm2 and the energy density of 3 J/cm2 are the optimal parameters for stimulating cell proliferation. Additionally, the results of sterilization show that blue light (415 nm) with an irradiance of 25 mW/cm2 and energy density of 50 J/cm2 can produce a significant effect on Pseudomonas aeruginosa. Moreover, the average error rates of irradiance are 1.93%, 1.73%, and 1.4%, which are associated with the irradiance of 5 mW/cm2, 15 mW/cm2, and 25 mW/cm2, showing the stability and robustness of the proposed system. Consequently, the uniformity of irradiance can achieve 97.9%, indicating that this work is capable of reducing variation in the experimental process. To enhance the universality of the treatment system for wound healing, this work additionally reveals a preliminary result of distinguishing the distinct wound area of a captured image using the RCNN-based model.

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