此篇論文主要探討極低頻電磁場，對小鼠黑色素腫瘤細胞內鈣離子以及細胞活性的影響。細胞活性主要是藉由MTT的方式來檢測。而細胞內的鈣離子濃度量測部分，則是先藉由fluo-4 AM的螢光染劑來染色，並使用高通量螢光顯微顯微鏡來拍攝並量測細胞內的鈣離子螢光值，此值和鈣離子濃度成正比。另外，鈣離子在細胞內扮演著極為重要的角色，主要為調控各種生物效應，像是細胞增生，細胞壞死，以及細胞凋亡等等。
而為了深入探討細胞內鈣離子的濃度，以及細胞活性的關係，我們所使用的實驗頻率為舒曼波頻率7.83±0.3Hz和五倍的舒曼波 頻率39.15±0.3Hz，搭載強度為0.3±0.05 mT的電磁場。另外，磁場暴露的時間長度為48小時。結果指出，極低頻電磁場可以增加細胞內鈣離子濃度。相較於控制組，五倍的舒曼波頻率39.15±0.3Hz對於細胞內鈣離子濃度提升的效果較好，其所提升的胞內鈣濃度的比率為20.9%，另外，舒曼波頻率7.83±0.3Hz對於細胞活性的抑制效果則較好，可以達到19.2%的抑制率。由結果可以得知，細胞內的鈣離子濃度，細胞生長活性的抑制率，存在一個窗口效應。

The paper presents the effect of extremely-low frequency (ELF) electromagnetic fields (EMF) on the intracellular calcium ions of B16F10 cancer cells (murine melanoma cancer cells), and cell viability. The cell viability was conducted by MTT assay. The intracellular calcium ions are dyed by fluo-4 AM and measured by high throughput screening (H.T.S) microscope instrument. As one of the important ions in the cells, the intracellular calcium ions play an important role on influencing several biological effects of cellular process, including cell proliferation, cell apoptosis, cell death and so on.
In order to verify the relationship between ELF-EMF and intracellular calcium ions, the Schumann frequency (7.83 ±0.3Hz) and the 5-fold Schumann frequency (39.15± 0.3Hz) with the uniform magnetic field intensity (0.3±0.05 mT) were chosen in the experiment .The exposure time of ELF-EMF is 48hr. The result shows that ELF-EMF can increase the intracellular calcium concentration of B16F10. The 5-fold Schumann frequency (39.15± 0.3Hz) owns the best consequence of increasing 20.9 % of ratio of intracellular calcium concentration compared to control groups. The Schumann frequency (7.83 ±0.3Hz) owns the best consequence of inhibiting 19.2% of the cell viability. The result indicates that the intracellular calcium concentration effected by EMF has the biological window effect that can regulate the cell fate.

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