在過往許多研究人員已指出電磁場與生物體之間互相作用的關係，因此為了更深入探討電磁場治療癌症的可能性，本篇論文主要朝著探討在時變ELF-EMF對於肺癌細胞A549的影響，在基於7.83Hz 掃頻區間加入間歇性暫停，磁場強度0.82mT下，進行不同開關時間點及多重複電磁場暴露，分別是開八小時關十六小時、開一小時關五小時以及結合上述兩者時間點的組合開關，實驗分別觀察24小時以及48小時。在結合不同環境參數下，使用MTT檢測的方式，觀察癌細胞的活性比率，從實驗結果上表明組合頻率開關下，第一天使用7.83Hz搭配暫停60Hz(開一關五)，第二天使用7.83Hz搭配暫停7.83Hz(開八關十六)，EMF對於癌細胞活性影響最大，有著15%的抑制率。因此我們後續有對此組合頻率進行分析，確定第二天使用的EMF有達到二次刺激的效果，後續有提出了一些假設，對於細胞活性的降低可能的原因為EMF造成細胞週期的改變，導致在G2 phase arrest，進而影響細胞存活率。

In order to investigate the potential of electromagnetic fields for cancer treatment, this paper focuses on the effects of time-varying ELF-EMF on lung cancer cells A549, with the addition of intermittent pauses at 7.83Hz sweep frequency intervals and a magnetic field strength of 0.82mT, and with different switching time points and multiple The experiments were conducted for 24 hours and 48 hours respectively, using a combination of 8 hours on and 16 hours off, 1 hour on and 5 hours off, and a combination of these two time points. The results of the experiment showed that the combined frequency switch, with a 7.83-pause-60Hz on the first day (on 1hr/ off 5hr) and 7.83-pause-7.83Hz on the second day (on 8hr/ off 16hr), had the greatest effect on the activity of cancer cells, with a 15% inhibition rate. Therefore, we subsequently analyzed this combination of frequencies and confirmed that the EMF used on the second day had achieved a secondary stimulatory effect. We then proposed some hypotheses that the decrease in cell activity could be due to the change in cell cycle caused by EMF, resulting in G2 phase arrest, which in turn affected cell survival.

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