脂質擁有複雜的結構且種類繁多，由於脂質與蛋白質的結合關係影響到許多生物功能途徑，所以探討脂質與蛋白質的結合關係已經是目前脂質體學中的重點之一；雖然以前因為技術上的困難，讓脂質-蛋白質的交互關係研究遠不及蛋白質與蛋白質和蛋白質與基因網路的交互關係研究，儘管如今技術的蓬勃發展，脂質的重要性被陸續重視，且脂質與蛋白質結合關係的研究興起，但大量的研究實驗數據卻沒有適當的資料庫把他們統整起來，這成了一道無形的門檻。雖然有脂質的資料庫有提供查詢與脂質相關的蛋白質，但他們資料都只是收集與脂質相關關鍵字的GO(Gene Ontology)和KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway，導致他們提供的資訊太少，也不是透過實驗去探討脂質與蛋白質的結合關係。因此我們收集了兩篇透過protein microarray和一篇透過lipid-array 研究脂質與蛋白質交互關係的實驗數據，經過整理後總共收集了32種脂質與 416種蛋白質有結合關係，且開發Yeast Lipid-Protein Binding Interaction Database (YLPBIdb) 提供生物學家一個搜尋脂質與蛋白質在酵母菌中結合關係的良好平台。在YLPBIdb我們提供使用者兩種搜尋模式(1)搜尋脂質會結到哪群蛋白質;(2)搜尋蛋白質會結合到哪些脂質。然後會將結果以網路圖的方式呈現，並且會進一步的做Enrichment分析，讓使用者可以更了解這群蛋白質群的生物特徵功能。我們還透過兩個實際例子來證明YLPBIdb的價值，(1) PI(3,4)P2、PI(4,5)P2、PI(3,5)P2和PI(3,4,5)P3這四種都是PI的磷酸化變形，在結構上只差一個磷酸根的位置，然而PI(3,5)P2與其他三種卻截然不同，這在YLPBIdb的Search By Lipid Combination可觀察得知；(2) 從YLPBIdb的Search By Protein Name 搜尋ROM2，發現它與PI3P、PI4P和PI(4,5)P2有結合關係，而有研究顯示ROM2確實會結合PI4P和PI(4,5)P2到並且影響到Stt4-Mss4激活Rho1 GTPase所調解MAP 激酶級聯反應。因此我們相信YLPBIdb能有助於研究脂質與蛋白質的交互關係。YLPBIdb網址：http://cosbi2.ee.ncku.edu.tw/YLPBIdb/

In recent years, evidence has emerged showing that the lipid and protein binding interaction involved in the intracellular signaling. Therefore, we understand that lipid and protein binding interaction plays an important role in biological function. However, there is no a database of lipid and protein binding interaction, so we collected three experimental data, two of which through protein microarray and one through lipid array assay , a total of the 32 kinds of lipids and 416 proteins binding interaction were collected. And we used these data to develop the Yeast Lipid-Protein Binding Interaction Database (YLPBIdb) to provide a good platform for biologists to search for the associative relationship between the lipid and protein in yeast. The YLPBIdb is available at http://cosbi2.ee.ncku.edu.tw/YLPBIdb/ .

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