近年來，許多研究指出極低頻電磁場(ELF_EMF)對細胞的生理現象有影響，例如代謝、移動、增殖以及凋亡。本篇論文應用3D立體電極捕捉細胞並利用電極陣列結合極低頻電磁場裝置，對癌細胞MDA-MB-231在ELF_EMF做及時、非侵入性的監控。在3D電極陣列裡，細胞阻抗量測長達12h，在這段時間，從阻抗量測可以發現，細胞經歷了三個階段，細胞蔓延、有絲分裂細胞變圓、細胞分裂。此外這篇研究也是第一個結合微管特性進細胞等校電路做數值探索，並且利用實驗結果對MDA-MB-231細胞在ELF-EMF與控制組的差異進行分析。實驗結果結合結合COMSOL軟體以及等校電路進行比較後，最終得到ELF-EMF對MDA-MB-231的影響。本篇研究認為ELF-EMF會抑制細胞微管的聚合，不僅進低了微管體積增加時的速率，在微管縮減時也造成了加速解聚的效果，因此ELF-EMF對微管的影響也影響到了癌細胞的功能，例如細胞蔓延以及延長有絲分裂。

In recent years, many studies have shown that extremely low frequency electromagnetic fields (ELF-EMF) influences cellular processes such as metabolism, motility, proliferation and apoptosis. In this study, three-dimensional cell capture and sensing array electrode (3D ECCSA) and an ELF-EMF device are integrated for real-time, non-invasive monitoring MDA-MB-231 cells under ELF-EMF. The cell impedance, activity and behavior are monitored by 3D ECCSA and microscope over a long period of time (12 h). In this period, MDA-MB-231 cell goes through three different stages: cell spreading, mitotic cell rounding, and cell division, which can be measured by changes in impedance. In addition, that is the first time that an equivalent circuit model of cell combined with microtubules, numerical investigation and the experimental results are utilized to analyze different status of human breast cancer cell MDA-MB-231 under ELF-EMF and control condition. The experimental results are compared with the numerical solutions from a circuit model of the single-cell by commercial COMSOL software package. Finally, relationship between cellular processes and ELF-EMF effect on MDA-MB-231 are explained by the electrical methods from the simulation and experimental results. This study suggests that ELF_EMF impedes the microtubules assembly, which slows down the increment of microtubules volume and accelerates the decrement of microtubules volume. As a result, the ELF_EMF influence on the microtubules of cancer cell may inhibit cellular function such as inhibiting cell spreading and prolonging mitosis.

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