此篇論文主要探討舒曼波對小鼠黑色素腫瘤細胞(B16F10)的細胞活性影響，並深入研究細胞內鈣離子以及電壓門控鈣離子通道(VGCC)的機制。本實驗使用舒曼波頻率為7.83±0.3Hz，搭載強度為0.3±0.05 mT的電磁場，磁場暴露的時間長度為48小時。實驗方式分為三個階段，先以舒曼波照射在B16F10細胞上，藉由MTT的方式來檢測細胞活性。第二部分是舒曼波照射後，量測細胞內的鈣離子濃度，藉由fluo-4螢光染劑染色，並使用高通量螢光顯微顯微鏡來拍攝鈣離子螢光值。第三部分使用VGCC阻斷劑，量測舒曼波對B16F10細胞內的鈣離子變化差異。鈣離子在細胞內扮演極為重要的角色，主要為調控各種生物效應，像是細胞增生，細胞壞死，和細胞凋亡等等。
　　結果指出，舒曼波對B16F10細胞照射48小時的抑制率達到25.5%。並可以增加細胞內鈣離子濃度19.2%。只照射30分鐘則可立即上升75%細胞內鈣離子濃度。上升75%鈣離子濃度中，發現有24%經由VGCC，51%經由細胞內鈣庫釋放。而24%經由VGCC上升的鈣離子濃度中，有9%是經由L-type VGCC，15%經由T-type VGCC進入。由此可知，舒曼波對B16F10細胞有抗癌的功效，其抑制原因和鈣離子關係密切。

This paper mainly discusses the effect of Schumann wave on the activity of mouse melanoma tumor cells (B16F10), and further studies the mechanism of intracellular calcium ion and voltage-gated calcium channel (VGCC).This experiment uses a Schumann wave frequency of 7.83±0.3 Hz and an electromagnetic field with an intensity of 0.3±0.05 mT. The magnetic field exposure time is 48 hours. The experimental method was divided into three parts. First, the B16F10 cells was exposed to Schumann wave, and cell activity was detected by MTT. The second part, after Shuman wave expose, the intracellular calcium concentration was measured, stained with fluo-4 fluorescent dye, and a high-throughput fluorescence microscopy was used to photograph the calcium ion fluorescence value. The third part, VGCC blockers were used to measure the differences in the calcium ion changes in B16F10 cells with and without Schumann waves exposure. As one of the important ions in the cells, the intracellular calcium ions play an important role on influencing several biological effects of the cellular process, including cell proliferation, cell apoptosis, cell death and so on.
The results show that the inhibitory rate of the B16F10 cells with the Schumann wave exposure for 48 hours reached 25.5%, and the intracellular calcium ion concentration increase 19.2%. Exposure for 30 minutes can immediately increase the intracellular calcium concentration by 75%. Of the 75% increase in calcium ion concentration, 24% was found via VGCC and 51% was released via intracellular calcium stores. Of the 24% increase in calcium concentration via VGCC, 9% were via L-type VGCC and 15% via T-type VGCC. It demonstrate that Schumann wave has an anti-cancer effect on B16F10 cells, and its inhibition could be related to calcium ions.

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