簡易檢索 / 詳目顯示

研究生: 黃承澤
Huang, Cheng-Tze
論文名稱: 7.83 Hz電磁場對肺癌細胞之分析
Analysis of Lung Cancer Cells by 7.83 Hz Electromagnetic Field
指導教授: 張凌昇
Jang, Ling-Sheng
學位類別: 碩士
Master
系所名稱: 電機資訊學院 - 電機工程學系
Department of Electrical Engineering
論文出版年: 2019
畢業學年度: 107
語文別: 英文
論文頁數: 45
中文關鍵詞: 極低頻電磁場肺癌細胞
外文關鍵詞: EMF, A549 cancer cells, Cell viability
相關次數: 點閱:42下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 肺癌是全球癌症死亡的最主要原因,大約80%的患者是肺癌中的非小細胞肺癌(NSCLC)。 NSCLC的主要治療方法是手術、化療、放射線治療和標靶治療。然而,這些治療伴隨著副作用,讓患有肺癌的患者仍然預後不良。
    許多研究人員已研究電磁場與生物相互關係,發現對癌細胞有生物學效應,並且暴露特定的頻率、暴露時間和磁場強度的電磁場,會讓癌細胞的細胞活性降低,產生抑制的現象。近年的大多提及是電磁場與細胞內鈣離子產生共振現象,影響癌細胞的生長,進而促使癌細胞走向凋亡。基於以上理由,我們嘗試用舒曼共振頻率7.83 ± 0.3 Hz結合不同電磁場的參數來探討其對肺癌細胞的影響。
    我們的研究結果顯示,肺癌細胞在舒曼共振頻率7.83 ± 0.3 Hz,線圈電流為51.4 mA,且切換暴露6小時持續兩天之後,發現對癌細胞有顯著的抑制現象,抑制率為88 %。此外癌細胞的存活率隨著磁場強度提高也有變化,我們推測此結果可能與鈣離子共振、電磁場的強度、細胞特異性和暴露時間的參數共同影響。

    Lung cancer is the most leading cause of cancer deaths in the world, and approximately 80 % of patients are non-small-cell lung cancer (NSCLC) among lung cancers. The typical treatments in NSCLC are surgery, chemotherapy, radiotherapy and target drug therapy. However, these treatments are accompanied with side effects, patients with lung cancer still have a poor prognosis.
    Many researchers have studied the relationship between electromagnetic fields and biology that have been founded biological effects on cancer cells. Exposure to specific frequencies, exposure times, and magnetic field intensity of EMF can reduce the cell viability and cause inhibition of cancer cells. In recent years, most of the theories have mentioned that EMF resonates with intracellular calcium ions, affecting the growth of cancer cells and promoting cancer cells to apoptosis. Base on above reasons, we use the Schumann resonance frequency of 7.83 ± 0.3 Hz that combined with the different parameters of EMF to explore the effect on lung cancer cells.
    Our results show that lung cancer cells are significant inhibition of cancer cells after being exposed parameters of coil loop current 51.4 mA at Schumann resonance frequency of 7.83 ± 0.3 Hz and switching exposure for 6 hours in two days. The cell inhibition rate is as high as 88%. Furthermore, the cancer cell viability changes with the increasing of magnetic field intensity. We speculate that the results may be influenced by parameters of calcium ion resonance, electromagnetic field intensity, cell specificity, and exposure time.

    中文摘要 .......I ABSTRACT.......II ACKNOWLEDGEMENT.......IV CONTENTS.......VI LIST OF TABLES.......VIII LIST OF FIGURES.......IX CHAPTER 1 INTRODUCTION.......1 1-1 Background and motivation.......1 1-1-1 Background.......1 1-1-2 Introduction of ELF-EMF.......2 1-1-3 Motivation.......5 1-2 Calcium ions influx.......7 CHAPTER 2 MATERIAL AND METHOD.......9 2-1 Cell culture.......9 2-2 EMF exposure system.......10 2-2-1 EMF device design.......10 2-2-2 Coil windings.......11 2-2-3 Signal type.......15 2-3 Cell analysis method.......16 2-3-1 MTT assay.......16 2-3-2 Optical density value (O.D value).......16 2-3-3 Statistical analysis.......17 CHAPTER 3 EXPERIMENTAL SETUP.......18 3-1 Experimental setup.......18 CHAPTER 4 EXPERIMENT RESULTS AND DISCUSSION.......21 4-1 Experiment results.......21 4-1-1 Experiment results of the Schumann frequency on A549.......21 4-1-2 Experiment results of the Schumann frequency on lung cells.......27 4-2 Discussions of experiment results.......31 CHAPTER 5 CONCLUSION.......38 REFERENCES.......40

    [1] Torre, Lindsey A., et al. "Global cancer statistics, 2012." CA: a cancer journal for clinicians 65.2 (2015): 87-108.

    [2] Coebergh, J. W. W., et al. "Serious co-morbidity among unselected cancer patients newly diagnosed in the southeastern part of The Netherlands in 1993–1996." Journal of clinical epidemiology 52.12 (1999): 1131-1136.

    [3] Baskar, Rajamanickam, et al. "Cancer and radiation therapy: current advances and future directions." International journal of medical sciences 9.3 (2012): 193.

    [4] Bryant, Rosalind. "Managing side effects of childhood cancer treatment." Journal of pediatric nursing 18.2 (2003): 113-125.

    [5] Crocetti, Sara, et al. "Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability." PloS one 8.9 (2013): e72944.

    [6] Barbault, Alexandre, et al. "Amplitude-modulated electromagnetic fields for the treatment of cancer: discovery of tumor-specific frequencies and assessment of a novel therapeutic approach." Journal of Experimental & Clinical Cancer Research 28.1 (2009): 51.

    [7] Evangelou, Angelos, et al. "Functionality of natural killer cells from end-stage cancer patients exposed to coherent electromagnetic fields." Electromagnetic biology and medicine30.1 (2011): 46-56.

    [8] Kirson, Eilon D., et al. "Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors." Proceedings of the National Academy of Sciences 104.24 (2007): 10152-10157.

    [9] Lacy-Hulbert, Adam, James C. Metcalfe, and Robin Hesketh. "Biological responses to electromagnetic fields." The FASEB Journal 12.6 (1998): 395-420.

    [10] Zhen, Yang, and W. O. Xing-de. "New Progress of Research of Biological Effect of Magnetic Fields [J]." Progress in modern biomedicine 9 (2006).

    [11] Simko, M., et al. "Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines." Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association 19.2 (1998): 85-91.

    [12] Lisi, Antonella, et al. "Extremely low frequency electromagnetic field exposure promotes differentiation of pituitary corticotrope‐derived AtT20 D16V cells." Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association 27.8 (2006): 641-651.

    [13] Xu, Yujun, et al. "Low frequency magnetic fields induce autophagy-associated cell death in lung cancer through miR-486-mediated inhibition of Akt/mTOR signaling pathway." Scientific reports 7.1 (2017): 11776.

    [14] Buckner, Carly A., et al. "Inhibition of cancer cell growth by exposure to a specific time-varying electromagnetic field involves T-type calcium channels." PLoS One 10.4 (2015): e0124136.

    [15] Catterall, William A. "Voltage-gated calcium channels." Cold Spring Harbor perspectives in biology 3.8 (2011): a003947.

    [16] Berg, Hermann. "Problems of weak electromagnetic field effects in cell biology." Bioelectrochemistry and bioenergetics 48.2 (1999): 355-360.

    [17] Crocetti, Sara, et al. "Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability." PloS one 8.9 (2013): e72944.

    [18] Filipovic, Nenad, et al. "Electromagnetic field investigation on different cancer cell lines." Cancer Cell International 14.1 (2014): 84.

    [19] Brighton, Carl T., et al. "Signal transduction in electrically stimulated bone cells." JBJS 83.10 (2001): 1514-1523.

    [20] Ross, Christina L., et al. "The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation." Stem cell research 15.1 (2015): 96-108.

    [21] Wang, Tingting, et al. "Involvement of midkine expression in the inhibitory effects of low‐frequency magnetic fields on cancer cells." Bioelectromagnetics 32.6 (2011): 443-452.

    [22] Nie, Yunzhong, et al. "Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation." BMC cancer 13.1 (2013): 582.

    [23] Te-Wei Yeh. Effects of Schumann Wave on B16F10 Cancer Cells. 2016

    [24] Tang, Jing-Yau, et al. "Effects of extremely low-frequency electromagnetic fields on B16F10 cancer cells." Electromagnetic biology and medicine (2019): 1-9.

    [25] Koziorowska, Anna, et al. "The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro." Przegląd Elektrotechniczny 5.93 (2017): 1.

    [26] Zimmerman, Jacquelyn W., et al. "Cancer cell proliferation is inhibited by specific modulation frequencies." British journal of cancer 106.2 (2012): 307.

    [27] Wang, Tingting, et al. "Involvement of midkine expression in the inhibitory effects of low‐frequency magnetic fields on cancer cells." Bioelectromagnetics 32.6 (2011): 443-452.

    [28] Kroupová, Jana, et al. "Low-frequency magnetic field effect on cytoskeleton and chromatin." Bioelectrochemistry 70.1 (2007): 96-100.

    [29] Buckner, Carly A., et al. "Exposure to a specific time‐varying electromagnetic field inhibits cell proliferation via cAMP and ERK signaling in cancer cells." Bioelectromagnetics 39.3 (2018): 217-230.

    [30] Buckner, Carly A., et al. "The effects of electromagnetic fields on B16‐BL6 cells are dependent on their spatial and temporal character." Bioelectromagnetics 38.3 (2017): 165-174.

    [31] International Commission on Non-Ionizing Radiation Protection. "Guidelines on limits of exposure to static magnetic fields." Health Physics 96.4 (2009): 504-514.

    [32] Walleczek, Jan, and Robert P. Liburdy. "Nonthermal 60 Hz sinusoidal magnetic-field exposure enhances 45Ca2+ uptake in rat thymocytes: dependence on mitogen activation." FEBS letters 271.1-2 (1990): 157-160.

    [33] Overwijk, Willem W., and Nicholas P. Restifo. "B16 as a mouse model for human melanoma." Current protocols in immunology 39.1 (2000): 20-1.

    [34] Das, A., et al. "Functional expression of voltage‐gated calcium channels in human melanoma." Pigment cell & melanoma research 25.2 (2012): 200-212.

    [35] Brighton, Carl T., et al. "Signal transduction in electrically stimulated bone cells." JBJS 83.10 (2001): 1514-1523.

    [36] Ćosić, Irena, et al. "Human electrophysiological signal responses to ELF Schumann resonance and artificial electromagnetic fields." FME Transactions 34.2 (2006): 93-103.

    [37] Lyle, Daniel B., et al. "Calcium uptake by leukemic and normal T‐lymphocytes exposed to low frequency magnetic fields." Bioelectromagnetics 12.3 (1991): 145-156.

    [38] Roderick, H. Llewelyn, and Simon J. Cook. "Ca2+ signalling checkpoints in cancer: remodelling Ca 2+ for cancer cell proliferation and survival." Nature Reviews Cancer 8.5 (2008): 361.

    [39] Prevarskaya, Natalia, et al. "Remodelling of Ca2+ transport in cancer: how it contributes to cancer hallmarks?." Philosophical Transactions of the Royal Society B: Biological Sciences 369.1638 (2014): 20130097

    [40] Berridge, Michael J. "Calcium signalling remodelling and disease." (2012): 297-309.

    [41] Taylor, James T., et al. "Calcium signaling and T-type calcium channels in cancer cell cycling." World journal of gastroenterology: WJG 14.32 (2008): 4984.

    [42] Pinton, Paolo, et al. "Calcium and apoptosis: ER-mitochondria Ca2+ transfer in the control of apoptosis." Oncogene 27.50 (2008): 6407.

    [43] Santini, M. T., et al. "Extremely low frequency (ELF) magnetic fields and apoptosis: a review." International Journal of Radiation Biology 81.1 (2005): 1-11.

    [44] 陈圆, et al. "钙离子通道和钙信号震荡模型研究 Modeling of Ca2+ Channels and Ca2+ Signal Oscillations." Biophysics 4.01 (2016): 1

    [45] Cho, Han-Wook, et al. "Pulsed electromagnetic fields stimulate cellular proliferation in different types of cells." IEEE Transactions on Magnetics 52.7 (2016): 1-4.

    [46] Lisi, Antonella, et al. "Extremely low frequency electromagnetic field exposure promotes differentiation of pituitary corticotrope‐derived AtT20 D16V cells." Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association 27.8 (2006): 641-651.
    [47] Teodori, Laura, et al. "Static magnetic fields affect calcium fluxes and inhibit stress‐induced apoptosis in human glioblastoma cells." Cytometry: The Journal of the International Society for Analytical Cytology 49.4 (2002): 143-149.

    [48] Denis Flipo, et al. Increased apoptosis, changes in intracellular Ca2+ , and functional alterations in lymphocytes
and macrophages after in vitro exposure
to static magnetic field.” Journal of toxicology and environmental health, part a, 54:63–76, 1998

    [49] Sergeev, Igor N. "Calcium signaling in cancer and vitamin D." The Journal of steroid biochemistry and molecular biology 97.1-2 (2005): 145-151.

    [50] Wojcik-Piotrowicz, Karolina, et al. "Cell viability modulation through changes of Ca2+-dependent signalling pathways." Progress in biophysics and molecular biology 121.1 (2016): 45-53.

    [51] Golbach, Lieke A., et al. "Calcium homeostasis and low-frequency magnetic and electric field exposure: A systematic review and meta-analysis of in vitro studies." Environment international 92 (2016): 695-706.

    [52] Azimi, I., S. J. Roberts‐Thomson, and G. R. Monteith. "Calcium influx pathways in breast cancer: opportunities for pharmacological intervention." British journal of pharmacology 171.4 (2014): 945-960.

    [53] Song, Kiwon, et al. "A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels." PloS one 13.7 (2018): e0199753.

    [54] Feng, Jianguo, et al. "Effect of adjuvant magnetic fields in radiotherapy on non-small-cell lung cancer cells in vitro." BioMed research international 2013 (2013).

    [55] Yao, Xueling, et al. "Effects of low-intensity nanosecond pulsed electromagnetic field on PC3 cells." 2013 Annual Report Conference on Electrical Insulation and Dielectric Phenomena. IEEE, 2013.

    [56] Zimmerman, Jacquelyn W., et al. "Cancer cell proliferation is inhibited by specific modulation frequencies." British journal of cancer 106.2 (2012): 307.

    [57] Han, Qi, et al. "Pre-exposure to 50 Hz-electromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells." PloS one 13.4 (2018): e0192888.

    [58] Kuan-Wei Chen. Inhibition of Human Breast Cancer Cell Viability by Modulating Low Frequency Electromagnetic Field Parameters. 2018.

    無法下載圖示 校內:2024-08-19公開
    校外:不公開
    電子論文尚未授權公開,紙本請查館藏目錄
    QR CODE