在最近三四年，由於人類基因解碼工作的完成，使生物晶片的研究開始受到重視。活體細胞晶片可以在細胞階層量測到多重細胞功能的參數，例如：細胞阻抗、O2、CO2、NO、葡萄糖濃度、pH值及基因表現數等，藉由這些參數可以有機會對細胞內複雜的反應過程進行更一步的了解。為因應此項研究的需求，本計畫進行細胞電阻抗的分析。現今的生物技術發展迅速，藉由醫學理論和工程的技術結合所開發的高靈敏度、高效率且即時和微量的生醫感應檢測裝置，已經有幾十年的研究歷史，其中細胞電阻抗分析(Cell electrical impedance analysis)的技術發展快速，成為現今的生醫檢測的有效方法之一。
本論文建立細胞阻抗分析平台，其中包含前端的細胞捕獲裝置以及AD5934開發板阻抗分析量測系統。細胞捕獲裝置目的在於利用MEMS之技術捕獲單一HeLa細胞，並且沒有其他電場或磁場等外力介入，可簡化量測分析。阻抗分析量測系統與PC整合可藉由資料擷取來建立細胞之等效電路模型。此不僅可應用於醫學檢測之快速分析應用於臨床試驗或是疾病檢測，還可省去大型機台的負荷和耗時。將來此平台亦可藉由與微型幫浦整合之方式成為Lab-on-a-chip支元件。

Since the completion of the sequencing of human genome will open up many new areas of study, scientists need to develop new research tools, approaches and capabilities in order to make this vast array of biological information to be useful. The growing availability of the genome sequence data is creating the possibility for developing a comprehensive understanding of complex cellular processes. In order to achieve this goal, it will be necessary to measure multiple parameters on chip in single living cells in real time, for instance, cell impedance, O2, CO2, NO, glucose, pH, and so on. The cell impedance changes when the ion concentration and potential of the cells are different. The impedance for single living cells can be used for fast disease diagnosis.
This work developed spectroscopy for the single cell impedance measurement. The spectroscopy contains a microfluidic device and an AD5934 Evaluation Board. The microfluidic device captures single cell capture physically by microstructures in the microchannel. The AD5934 Evaluation Board and GUI (Graphic user interface) software could measure the impedance of a single HeLa cell (Human cervical epithelioid carcinoma). The device includes a glass substrate with electrodes and a PDMS channel with micro pillars. The commercial software CFD-ACE+ was used to verify the flow of the microstructures in the channel. Additionally, an equivalent circuit model of the device was established and fitted well to the experimental results in this study.

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