本論文提出一個光學與電阻抗式可攜式血液凝固檢測儀，可準確地量測微量全血之凝固時間；並且結合無線傳輸應用於遠距離照護及監測，使病患可隨時隨地進行凝血時間的檢測。目前醫用及市面上的偵測凝血儀器價格昂貴，不適合個人購買及居家使用，因此我們提出並實作完成一套使用光學檢測，可直接偵測全血的儀器。
本儀器使用透射光法可藉訊號一階微分之最大加速點準確判斷出凝血時間。光檢測系統使用德州儀器生產之單晶片(MSP430F149)進行控制、類比數位轉換、與數位信號處理，並經由光檢測元件、類比濾波、數位濾波以優化檢測結果。另一方面，我們同時採用另一個常見之檢測方法進行比較，電阻抗分析法使用阻抗分析儀由一階微分判斷凝血時間。
為了驗證系統的可重複性，進行40次實驗，變異係數(Coefficient of Variation, CV)為2.29%，證實系統檢測結果重複性高。臨床實驗使用167管全血，每管重複兩次於攝氏37度之溫室進行實驗，實驗結果取第一次結果進行統計，由臨床實驗結果可驗證本自製儀器具有高度之準確度，與大型儀器(ACL TOP)比較相關係數(R)達0.956，並已經過167組實驗驗證，證明系統穩定性。藉由本論文研究得知凝血訊號受RBC、Fib、Hct的影響，並可由凝血訊號與CBC數據進行統計分析，結果顯示第一次實驗及第二次實驗的最大加速點斜率與Fib有高度相關性。本論文所提出之凝血檢測儀具有可攜式、高準確度、成本低廉、使用簡單、直接偵測全血、並使用藍芽無線傳輸等優點，可達成遠距離照護以及降低社會醫療成本的優勢。

This thesis presents a portable blood coagulation detector design by using optical and impedance methods, which can measure whole blood coagulation time accurately. In addition, we integrated the wireless transmission technologies with the proposed detector for long-distant caring and monitoring applications. This detector can detect the coagulation time at any time and any place for point-of-care testing (POCT). The cost of blood coagulation detector in hospital and commercial products are very expensive, which is not suitable for personal use or home use. We provide and fabricate a whole blood coagulation detector using the optical method.
This instrument is based on the method of light transmittance to measure precisely the coagulation time according to the maximum accelerated point from the first-order differential curves. The optical detection uses the microprocessor TI MSP430F149, which includes 12-bit analog-to-digital converter (ADC) to control, to convert signals, and perform digital signal processing. By using high sensitivie optical-detecting sensors, analog filters, and digital filter, the detecting results can be optimized. Meanwhile, we use another common impedance method to compare. This method uses the impedance analyzer to determine the coagulation time according to first-order differential curve. This thesis investigates the optimal frequency for impedance method.
To verify the reliability in this system, we performed the experiments for 40 times and the results are repeatable with the coefficient of variation (CV) =2.29%. The clinical experiments are based on 167 tubes of whole blood, and each tube is repeated twice in warm house at 37 Celsius degrees. We took the result of the first time to do statistic analysis. From the clinical experiment results, the accuracy and stability of this instrument were validated by testing 167 experiments. The correlated coefficients of the prothrombin time (PT) are 0.956 between the instrument and the hospital blood analyzer (ACL TOP).
This thesis also dicover that the coagulation signals are affected by RBC, Fib, and Hct, and statistics between the coagulation signals and CBC are performed. The result shows that the slope at the maximum accelerated point in the first and second experiments are highly related with Fib。Thus, this device has advantages of portability, low cost for commercial mass marketing, easy operation, straightforward detection of whole blood and Bluetooth wireless transmission for long distance health care.

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