現代人由於工作忙碌，常常累積許多壓力，進而產生負面的情緒，長時間之後往往會產生憂鬱、躁鬱、失眠等症狀。相關研究指出，靜坐練習及深呼吸在生理、心理各方面皆有顯著的放鬆效果。
本研究整合了心電圖(ECG)與肌電圖(EMG)的量測，並將所產生的潘凱圖(Poincare Plot)分析，應用於情緒分析。潘凱圖擁有簡單運算和適合短時間資料分析的優點。藉由心電圖所產生的心率變異度分析(HRV)可用以了解自律神經活性的平衡狀態；而肌電圖可用以分析受測者臉部肌肉的變化。運用本研究所建立出的情緒反應分析系統，並利用匹斯堡睡眠品質量表(PSQI)與生活健康量表(SF-36)，篩選出條件符合的健康受測者來進行實驗。藉由圖片給予受測者不同的情緒刺激，分別量測各種生理參數的變化。最後再加上靜坐練習和深呼吸兩種身心放鬆方法，來觀察其對於情緒起伏的影響。初步結果顯示，在觀看較高刺激性的圖片時，潘凱圖中的SD2、SD2/SD1明顯較高，代表交感神經活性指標升高；而當受測者接受靜坐練習及深呼吸後，SD2明顯比未經此兩種放鬆方法前高，代表副交感神經活性升高，並且情緒也較不容易因受到刺激而起伏波動。

In the modern time, people are easy to accumulate mental stress because of their busy work. As a result, they often have negative emotions, which in turn may cause the disease depression, manic depression, insomnia and other symptoms after a long time. In the previous studies, meditation and deep breathing exercises can have a significant effect on your physical and mental relaxations .
In this study, we have integrated electrocardiogram (ECG), electromyogram (EMG), and also use Poincare Plot to analyze the emotional responses. Poincare Plot has the advantages of having simplified calculation process and being very suitable for a short time data analysis. By using HRV based on ECG, we can understand the balance of autonomic nervous activity, and EMG can be used to measure the contraction and relaxation of participants’ specific facial muscles. By using the self-designed emotional respose analysis system and applying the Pittsburgh Sleep Quality Index (PSQI) with Life Health Questionnaire (SF-36), we can select the healthy subjects who meets the requirements of our experiments objectively. In our research, we measured the related physiological responses of the subjects by stimulating them with different pictures. We also observe the effects of meditation relaxation exercises and deep breathing on the emotional responses of the subjects.
Finally, we found that the SD2,and SD2/SD1of Poincare Plot show higher outcome when watching more provocative picture. It indicates the increase of sympathetic activity. And after the subjects completing meditation practice or deep breathing exercises, the SD2 is significantly higher than before, and it represents that the emotional responses are not vulnerable to the interference of environmental stimulation.

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