在當今生活步調快及壓力大的社會中，飲食問題常是現代人所忽略的，也導致罹患糖尿病的人口逐年增加，為了減緩及預防此疾病，許多學者及相關研究提出了以偵測醣化血色素的濃度，即可達到準確偵測及預防的此疾病的功用，與過往偵測血糖最大的差異在於，血糖易受到一天的飲食、情緒甚至是服用的藥物所影響，導致結果不準確，而偵測醣化血色素最大優勢在於準確且不受短暫的變因影響，但現今偵測醣化血色素的相關儀器仍有成本高、攜帶不易及操作不變的問題，因此對於低成本、製作容易的可攜式晶片開發是不可或缺的一個議題。
　　為解決相關儀器成本高及攜帶不易的問題，在本研究中，使用一低成本的可攜式的阻抗量測系統及環型指叉式電極達到偵測醣血色素比例的目的，一般人醣化血色素比例約為4.5%~6.5%，大於7%即有可能成為糖尿病患者，為達到偵測的目的，使用自主性單分子層及蛋白質與其間的交互作用固定蛋白質，而電場通過的多寡會受蛋白質附著的量所影響，使用等校電路的模型即可計算蛋白質附著前後的阻抗變化量，在本研究中，提出蛋白質覆蓋於電極上的面積對阻抗變化的影響之理論，搭配微流道將包含醣化血色素的血色素溶液經由多個電極作分離後，對各電極作阻抗量測，將量測的變化量阻抗代入本研究所提出之理論，即可得知各電極上的蛋白質覆蓋面積，最後加總各分離電極的覆蓋面積，不同比例的醣化血色素即可經由此方式偵測出來。
　　本研究使用可攜式阻抗量測系統及環型指叉式電極，搭配上所提出的理論，成功檢測出5%~15%的醣化血色素。因改善了量測儀器的攜帶不易及成本高的缺陷，且僅需阻抗量測即可辨別醣化血色素的比例，故本研究已相當接近實際的臨床需求。

　　In order to control and detect diabetes, some scholars and researchers had pointed out glycated hemoglobin (HbA1c) is one of the diagnostic assays to reflect the glucose level for long-term monitored. To improve the drawbacks of instruments for HbA1c detection, such as large, bulky and difficult to operate, a portable device is proposed to detect the ratio of HbA1c to Hb; with ring-shaped interdigital electrode based on impedance measurement. It is label-free, low sample consumption and no other reagent is required while the measurement process, because this is an on-chip biosensor.
　　The ring-shaped interdigital electrode is first modified with self-assembled monolayer to immobilize HbA1c, then measure the impedance caused by protein through portable device, and calculate before-after impedance deviation through the electrical model. A theory of the impact of protein coverage to impedance deviation is proposed in this study. By separating the HbA1c in Hb on ten separation electrodes with microfluidic channel, and measure the impedance deviation for ten electrodes, finally, the area of protein covered can be obtained through the theory proposed in this study. The different ratio of HbA1c to Hb can be figured out by summing the area of protein covered on each separation electrodes.
　　The 5% to 15% ratio of HbA1c to Hb are successfully detected through the portable device and intedigital electrode in this study. Because not only the drawbacks of large and bulky for commercial instruments are improved, and is also easy to determine the ratio of HbA1c to Hb with impedance measurement which is a simple way to analyse, it is close to the clinical needs extremely of this research.

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