細胞接觸基質或材料時，細胞首先發生貼附及黏著的現象，這個現象會決定細胞在基材上的生理表現，進而影響細胞所能發揮的特有功能，因此有學者藉由評估細胞的黏著能力，來評斷細胞的表現。在這些研究中有許多的報告從生化、材料、力學的觀點來評估細胞黏著表現。本研究從力學的觀點，來評估細胞的黏著能力。
本研究的目的為設計一套機構，並利用此機構量測單一細胞的黏著力量，藉此評估細胞黏著能力。機構中採用原子力顯微鏡探針上的懸桿為施力為施力設備，藉由細胞以等速逼近懸桿，使懸桿對細胞施加剪力並將細胞從基材上刮下，再分析懸桿的偏移量求出探針對細胞的施力，藉此求得細胞的黏著力量。
完成機構設計及測試後，利用此機構觀察3T3纖維母細胞在不同溫度及實驗溶液下，細胞黏著力量的改變。結果發現細胞在相同溫度下於Dulbeccos Modification of Eagles Medium（DMEM，培養基）+10％Fetal Bovine Serum（FBS，胎牛血清）中的黏著現最佳，其次為在DMEM中，而在Phospate-Buffered Saline（PBS，無機鹽溶液）中的表現則最差。此外發現細胞在25℃時的黏著力較37℃佳，推論造成此結果的因素為加熱片由培養皿底部加熱，造成培養皿上的細胞所在溫度較37℃高，造成細胞和基材間的黏著能力下降。

After cells attach the substrata, cells begin to spread and form an organized actin cytoskeleton and complex transmembrane signaling regions, then cells start to express their functions, such as proliferation, differentiation and so on. Therefore cell adhesion is very important. Recently, a number of studies have investigated cell adhesion from the viewpoint of biochemistry, materials, and mechanics. In this study, we observed the adhesion from mechanical point of view.
The objective of this study was to develop a detachment technique to quantitatively measure the adhesive force between cells and substrata. The detachment of a cell was completed by a custom-designed rig which is consisted of the probe of AFM (atomic force microscopy), an AFM probe holder, and a cantilever arm mounted on the laser tweezers workstation. The cell is moved using a motorized stage to contact the AFM probe and the cell adhesion force is measured in terms of the deflection of the probe by video image analysis.
Finally, this developed detachment technique was used to measure the adhesion force of 3T3 fibroblast in different mediums at temperature 25℃ and 37℃, respectively. The results demonstrate that a greater force is required to detach cells in Dulbeccos modification of eagles medium (DMEM)+10％ fetal bovine serum (FBS) versus DMEM and PBS (Phospate-Buffered Saline). However, our result also showed that the adhesion force of 3T3 fibroblast in 25℃ is greater than in 37℃. We infer that the heater which transfers heat directly from the bottom of the petri dish may cause the poor uniformity of temperature inside. And the temperature at the contact area where cells grow on may be much higher than 37℃ such that the possible damage to the cells will decrease the adhesion force of cells to substrate.

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