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研究生: 柯銀隆
Ke, Yin-Lung
論文名稱: 低頻脈衝電磁場激發抗生素電磁訊號對大腸桿菌生長影響之探討
Influence of Electromagnetic Signal of Antibiotics Excited by Low Frequency Pulsed Electromagnetic Fields on Growth of Escherichia coli
指導教授: 張凌昇
Jang, Ling-Sheng
學位類別: 碩士
Master
系所名稱: 電機資訊學院 - 電機工程學系
Department of Electrical Engineering
論文出版年: 2011
畢業學年度: 99
語文別: 英文
論文頁數: 33
中文關鍵詞: 能量醫學脈衝電磁場電磁訊號大腸桿菌
外文關鍵詞: Energy Medicine, pulsed electromagnetic fields, electromagnetic signal, Escherichia coli
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  • 能量醫學提供病人一個新的醫療選擇,它能對疾病做非侵入性的檢測並且提供病人一種非藥物的治療方法。在本論文中,將大腸桿菌藉由能量醫學的理論應用至微弱且極低頻的脈衝電磁場上。電磁訊號耦合儀利用由螺線管線圈所產生的脈衝電磁場來激發抗生素的電磁訊號,且將它應用於大腸桿菌上。實驗藉由將大腸桿菌暴露在含有抗生素電磁訊號的脈衝電磁場中來研究抗生素電磁訊號對大腸桿菌的抑制效果。抗生素電磁訊號對大腸桿菌的抑制率在對數期為20%,而在穩定期則為10%。在對數期與穩定期中,抗生素電磁訊號的抑制率分別比脈衝電磁場的抑制率高14%與4%。實驗結果證明電磁訊號耦合儀成功的將抗生素的電磁訊號作耦合。此外,可以藉由在大腸桿菌的不同生長期中施予不同的載波頻率將抗生素電磁訊號的抑制率增高。

    Energy Medicine (EM) provides a new medical choice for patients, and its advantages are the non-invasive detection and non-drug treatment. In this study, EM is applied to Escherichia coli (E. coli) with weak extremely-low-frequency pulsed electromagnetic fields (PEMFs). PEMFs are produced by solenoidal coils for exciting the electromagnetic signal of antibiotics. The electromagnetic signals of antibiotics can be coupled by using electromagnetic signal coupling instrument and applied to the E. coli cells. The efficacy of the electromagnetic signal of antibiotics is investigated by exploring the inhibition rate of E. coli cells cultivated in the exposure to PEMFs with the electromagnetic signal of antibiotics. The inhibition rates of the electromagnetic signal of antibiotics in the logarithmic and stationary phase are about 20% and 10%, respectively, which are higher than those without the electromagnetic signal of antibiotics. The results demonstrate that the electromagnetic signal of antibiotics is successfully coupled by the electromagnetic signal coupling instrument. In addition, the inhibition rate of the electromagnetic signal of antibiotics on growth of E. coli in the stationary phase can be increased by changing the carrier frequency. This phenomenon shows that E. coli may have drug resistance to antibiotics.

    中文摘要 I ABSTRACT II Acknowledgment III CONTENTS IV LIST OF FIGURES VI Chapter 1 INTRODUCTION 1 Chapter 2 MATERIAL AND THEORY 4 2.1 Molecular signaling 4 2.2 Electromagnetic signal coupling instrument 6 2.3 Optical density value 12 CHAPTER 3 EXPERIMENTAL SETUP 14 3.1 Experimental setup 14 3.2 Exciting condition 16 3.2.1 Electromagnetic signal of antibiotics on growth of E. coli 16 3.2.2 PEMFs on growth of E. coli 16 3.2.3 Changing the carrier frequency of the electromagnetic signal of antibiotics 17 3.2.4 Changing carrier frequency in the different phases of E. coli growth 18 CHAPTER 4 EXPERIMENTAL RESULTS 19 4.1 The electromagnetic signal of antibiotics on growth of E. coli 19 4.2 PEMFs on growth of E. coli 20 4.3 Changing the carrier frequency of the electromagnetic signal of antibiotics 21 4.4 Changing carrier frequency in the different phases of E. coli growth 22 CHAPTER 5 DISCUSSION 24 CHAPTER 6 CONCLUSION 30 References 31

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