一氧化碳可抑制細胞內粒線體活性，使其發生細胞凋亡。本篇研究選擇以高生物相容性之鈷鐵普魯士藍奈米立方體，於酸性環境下發生金屬置換反應，而產生出近紅外光波段吸收。接著，將乙二胺分子與材料表面之鐵離子進行配位螯合，使其表面帶有胺基用於將帶有羧基之一氧化碳釋放分子401修飾於表面上。合成出攜帶一氧化碳之鈷鐵普魯士藍奈米籠後，利用材料於近紅外光波段有明顯吸收之特性，材料以808 nm近紅外光雷射照射進行光熱轉換達到升溫效果，進而調控一氧化碳分子的釋放。材料結合一氧化碳氣體與光熱治療消滅癌細胞，在釋放一氧化碳的同時，原本於一氧化碳釋放分子401當中之一價錳離子會被氧化形成二價，用於增強磁振造影可做為腫瘤診斷之用途。

Carbon monoxide (CO) can inhibit the activity of mitochondria causing cell apoptosis. Herein, we design a controllable carbon monoxide releasing platform, a CO releasing molecules conjugated cobalt Prussian blue nanocages (hCPB). First, we synthesize the cobalt Prussian blue nanocubes and further treat with acid for metal replacement reaction. After the acidic corrosion, it shows the increase of near infrared (600-900 nm) absorbance as prolonged reaction time. The Fe sites on surface was chelated with ethylenediamine to yield amine-functionalized hCPB NPs followed by conjugation with CORM-401 by means of amide formation. The CORM-401 derivatized cobalt Prussian blue nanoacage exhibits NIR-responsive hyperthermia effect giving the release of CO. In our expectation, the combination of CO and photothermal therapy displays synergistic effect against cancer cells. Moreover, the Mn1+ may oxidized to Mn2+ concomitant of CO release and provide the T1-weighted MR imaging for tumor diagnosis.

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