單一細胞的操控一直是許多生物工程上的核心技術，此種核心技術對於許多更進一步的研究都是必要的，其中關於細胞阻抗分析的研究對於了解細胞的生理情形是一種很有效率的生物量測技術。因此，本研究將單細胞操縱與捕捉系統和量測技術做一個整合。本論文設計了捕捉和量測電極結合的生物晶片，利用電熱和負介電泳效應產生的控制方式將細胞或粒子準確地移動至量測電極再進行阻抗量測。負介電泳和電熱效應是電性操作方法中的一種，而且能夠與細胞捕捉後的檢測、分析等技術作結合。在過去研究中的微型捕捉電極在此研究中分開而成為一組量測電極。除此之外，為了更精準地將細胞固定在量測電極上，結構的尺寸和形狀必須更嚴謹的設計。我們利用模擬和分析找出最適用的結構參數，並且透過分析找出結構參數和捕捉範圍的關係讓此結構可更一步延伸使用至不同大小的細胞。我們亦透過反覆抓取的阻抗量測分析以驗證定位的準確度，其誤差低於3％，這結果也符合預期證明了此結構定位的可靠性。最後，此結構也實現在HeLa細胞的捕捉和量測。

The research of individual cells is an important technique in many kinds of biological study. In addition, cell impedance analysis is an effective way of biological measurement. Therefore, integrating the measurement technique into the single-cell trapping structure is the aim of this work. This study presents a cell manipulation and measurement microchip to achieve a precise positioning which uses the Alternating current electrokinetics (ACET) and negative dielectrophoresis (nDEP) force to move the particle and cell on the measurement electrodes. ACET and DEP are the electrical methods to manipulate particle, and can be easily combined with subsequent analyses based on electric fields. The microwell in the prior study is separated into two parts and regarded as the measurement electrodes. Besides, the original structure has been modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the parameters in the structure. By means of simulations and analyses, the optimum structure for the cell is presented. In addition, taking the advantage of analyses can interpret the capture range so that the structure is selective for cells of different sizes. To demonstrate the precision of positioning, the experiment capturing and measuring the particle repeatedly was implemented and the result showed the function of positioning is lower than 3％ and reliable. Finally, the structure was applied to trap and measure the HeLa cell.

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