文獻中指出環境中真菌含量能引起呼吸道疾病，因此能正確定量環境中的真菌濃度對於環境衛生評估是很重要的。
目前評估真菌濃度是以培養基培養分析法將採集所得之真菌於培養基中培養，再計數在培養基中之菌落數，此法耗時，且其真菌存活率受培養環境及所選擇之培養基之影響，且菌落計數亦非準確之數量化方法；因此所得之濃度會與真實情況有差異。

有鑑於以往用培養基培養分析法來分析真菌之濃度之效果不理想；故期望能發展更精確之方法來測量真菌之濃度。麥角質醇是真菌細胞壁上的主要化學成分，真菌的總數和細胞壁上的麥角質醇含量成對應關係，故可測麥角質醇的含量來推算真菌的總量。此法優點在於可以現代之儀器來進行分析，而使分析時間縮短，亦可提高分析結果之準確度。但因目前此法之靈敏度無法提高，至不符現實需要。

因HPLC-FLUO之靈敏度比HPLC-UV為佳，且分析樣品的極性範圍比GC廣，故將麥角質醇衍生化，再以HPLC-FLUO來分析，將可提高化學分析法之靈敏度。
以9-anthracene-carboxyl chloride當衍生化試劑與麥角質醇反應，反應條件為室溫下，於手套箱中，將9-anthracene-carboxyl chloride溶液0.2mL（9-anthracene-carboxyl acid濃度為 0.1g/mL）加入裝有麥角酯醇的樣品瓶中，搖動均勻，反應1小時，，經C18固相萃取管柱初步純化，濃縮後過濾以HPLC-FLUO分析，螢光偵測器之激發波長設定為252nm，放射波長設定為465nm。在濃度範圍29.1～1470ppb間可得一檢量線，檢量線方程式為y=646.06x-30592。方法偵測極限為46.5ppb。因最後上機之注射量為0.1mL，因此換算成重量為4.65ng。

以此方法應該可以提高使用化學法分析環境中真菌方法的靈敏度，以及降低偵測極限。但尚須克服衍生物螢光強度不夠，以及干擾過多的現象。

The environmental fungal exposure has been suggested to be associated with several respiratory diseases. Therefore it is important for evaluating public health in quantitative analysis of airborne fungal.

The traditional method, estimate colony-forming unit (CFU), determinates exposure fungal after proper incubation. However, the viability and culturability of fungi is time-consuming and depends heavily on environmental conditions and the types of culture medium chosen. It is a semi-quantitative method. Therefore the method cannot present real condition. So, it is essential to develop more accurate quantitative method to determinate fungal. Ergosterol is one of the major compound on fungal cell, and the amount of fungi is related to the level of ergosterol. Therefore, analyzing ergosterol could determinate the total fungal. It can shorten analysis time, and make the result more accurate. However, the sensitivity of the method is still too low. The sensitive of HPLC-Fluo is more than HPLC-UV. Therefore, this study proposes to the sensitivity by analyzing airborne fungal.

9-anthracene-carboxyl chloride, a Fluorescence derivatising reagent, reacts to ergosterol. A 0.2mL volume of 9-anthracene-carboxyl chloride was added to sampler vial with ergosterol in the glassbox. The vial were closed and allowed to react for 1 hour at room temperature after vigorous shaking. The reaction mixture was purified by RP 18 cartridge, and the fluorescene-derivatized compound was condensed, and then filtered. The solution was employed directly for HPLC quantitation, fluorescence detection at excitation (Ex)=252 nm and emission (Em)=465 nm. The standard curve was established at ergosterol concentrations from 29.1 ppb to 1470 ppb. The standard curve equation is y=646.06x-30592. The method detection limit is 46.5 ppb, and the injection volume is 0.1 mL, so it can be converted to 4.65 ng.

The method should improve the sensitivity and lower the detection limit to analyze airborne fungal, but we firstly required to overcome the problem which fluorescence signal intensity of fluorescene-derivatized compound is too weak and the interferences are too much in our study.

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