Trametes (栓菌屬) belongs to medically acclaimed family Polyporaceae and includes nearly 195 species. Members of this genus are widely distributed in various biotas throughout the world and have a tradition of use in Chinese medicine for thousands of years. It is considered useful in many aspects of healing, improvement of organ function, energy increase, and so forth. Among the confirmed biological activities, antioxidant, antimicrobial, and anti-inflammatory are the most often mentioned.

Trametes cubensis (立方栓菌) and Trametes suaveolens (香栓菌) are commonly used edible fungi in Vietnam and belong to the Trametes genus. They mainly grow on living or dead trees. The fruiting bodies of these fungi display various shapes, white color, and are types of saprophytic fungi. T. cubensis exhibited strong anti-HIV-1 activity. In addition, T. suaveolens demonstrated antioxidant, anti-complementary, and anti-HIV-1 bioactivities. However, the relationship between constituents of T. cubensis and T. suaveolens with anti-inflammatory bioactivity was still not clearly established. In the preliminary examination, the methanol extracts of fruiting bodies of T. cubensis and T. suaveolens were subjected to evaluation for their anti-inflammatory bioactivities. The results that T. cubensis promoted superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils, indicated the immunostimulatory effect. Therefore, the present study is aimed to purify and identify the bioactive principles of T. cubensis and T. suaveolens. Totally twenty compounds were isolated from the fruiting body of T. cubensis and T. suaveolens, and their structures were identified through the comparison of their physical and spectroscopic data with those published in the literature. Among these isolates, some compounds were potential according to the literature reports. The purified constituents will be subjected to evaluate their anti-inflammatory bioactivity by human neutrophil model.

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