本研究先調查高中自然科教師對於探究實作課程的態度，以台灣中部某一所高中的27位自然科教師為樣本，包含物理科、化學科及生物科。結果顯示教師們認為施行探究實作課程最主要的障礙是需要更多的時間，除此之外，亦擔心探究實作課程之教學法無法適用於所有的學生、設備不足以及自身知識不夠，無法充分發展探究實作課程。並且比較偏鄉地區及城市地區高中生對於生物科技知識與態度及農業生技之接受度是否有差異，偏鄉地區學校為台灣南部某一所高中的23位學生為樣本（平均年紀17歲），城市地區學校為台灣中部某一所高中的42名學生為樣本（平均年紀16歲），結果顯示城市地區的學生比偏鄉地區的學生，更能接受動物的基因工程。為了解決教師們認為探究實作課程需要花費更多時間的問題，因此設計一個簡易的探究實作課程教案，進而比較探究實作課程與傳統式課程對於高中二年級學生的學習有效性，以城市地區學校的42名學生為樣本，學生分為城市地區學校A班（N＝22）和城市地區學校B班（N＝20）。城市地區學校A班使用本實驗所設計之DNA粗萃取實驗模組結合探究教學活動，而城市地區學校B班則使用傳統式的課程活動實施相同實驗。研究結果顯示，相較於傳統式的課程活動，實施探究實作課程活動的學生在理解植物基因改良，以及相關的生物科技知識及態度能有較顯著提升。此研究結果說明，利用探究實作課程更能有效提升學生的學習成果。

The researcher investigated the attitudes of science teachers in high school toward the inquiry-based curriculum. There were 27 science teachers as participants. The results showed that teachers’ major difficulty with the implementation of inquiry-based curriculum was the need for more time than before. Next, the researcher compared the difference between two groups of students in rural and urban areas about the biotechnology knowledge, attitudes, and acceptance of agricultural biotechnology. 23 students were participants in a high school from the rural area, and 42 students are participants from the urban area. The results showed that students in urban areas are more acceptable to genetic engineering of animals than those in rural areas. A simple inquiry-based curriculum was designed to further compare the learning effectiveness of the high school students to inquiry-based curriculum and the traditional one. 42 students from urban areas were participants and divided into Class A and Class B. Class A applied the DNA rough extraction experiment of inquiry-based curriculum; while class B used the traditional curriculum to implement the same experiment. The results of this study showed the students who implemented inquiry-based curriculum were capable of understanding more about plant genetic modified and related biotechnology knowledge and attitude than those who were lectured with the traditional curriculum.
Key words: inquiry-based curriculum, rural area, and acceptance of agricultural biotechnology

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