本篇論文是利用電阻抗分析方式去分析細胞的生長代謝狀態，細胞型態的不同會影響細胞的電阻抗，進而電阻抗的差異便成了細胞生長代謝的指標。本篇論文利用微流道方式結合平面電極做單細胞的捕捉、培養以及阻抗量測。實驗分為溫度對細胞之影響以及牛樟芝對細胞之影響。細胞死亡方式大致分為兩種，一種是細胞凋亡一種是細胞壞死，細胞凋亡會造成細胞萎縮，細胞膜皺褶但細胞膜為完整狀態；細胞壞死則會造成細胞膜之破裂，細胞核之腫脹，細胞間質會洩出於胞外。細胞凋亡會自然地發生，但細胞壞死則是細胞遇到創傷才會引發之機制。本實驗是加入牛樟芝看細胞之生長代謝狀況，而牛樟芝對於細胞之效果是使細胞凋亡而非細胞壞死，本實驗結果表示，有加入牛樟芝的細胞有明顯地縮小相對於沒有加入牛樟芝的情況而言，而此結果顯示出牛樟芝對此細胞造成細胞凋亡而非細胞壞死。然而針對此實驗做差異度的分析，有加入牛樟芝的細胞之差異度明顯比沒有加入牛樟芝的細胞還來的高，此也應證了有加入牛樟芝的細胞會萎縮的嚴重相對於沒有加入牛樟芝的細胞來說。最後此實驗說明了，利用電阻抗方式可以快速的檢測單細胞的生長代謝狀態，以細胞凋亡之角度來看。

The electrical impedance analysis of single cell can provide the information of cells’ pathological condition on the different environment. The electrical properties are affected by the measurement environment such as the cell location, the cell adhesion, the cells’ size and so on. This paper observes the impedance variation in different cells cultural condition for the single melanoma cell. The proposed microfluidic can capture single cell, maintain the cell normal growth condition and, measure the single melanoma cell impedance by impedance analyzer and function generator. Additionally, the rate of impedance variation (ROIV) may be used to know the cell grow condition. Cellular apoptosis will affect cell size and membrane surface area. They can even affect the electrical properties of cells. Therefore, the cell equivalent circuit model is established tofit the results. The ROIV is 15.23 %, 17.04 % and 12.60 % at the 24 hr in the temperature range of 34 ℃, 37 ℃, 40 ℃, respectively without Antrodia cinnamomea (AC) adding. The ROIV is 17.04 %, 40.37 % and 45.02 % at the 24 hr in 37 ℃ with the 0 μl/ml, 20μl/ml and 40μl/ml AC adding, respectively. The results show that the cell impedance variation of cells which are cultured without the AC is much lower than the cells which are cultured with the AC. At the respect of cellular morphology, with the AC adding, the cells shrink obviously among 24 hours, and without the AC adding, the cells may shrink barely. Thus, the study presents the way to observe the cell condition by electric analysis.

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