根據醫學報導，糖尿病患者呼出氣體中所含的丙酮濃度高於一般正常值，故可將丙酮視為糖尿病診斷的指標，取代侵入式傳統抽血方法。因此，本研究利用厚膜印刷及電鍍方法製備工作電極，配合固態高分子電解質防止液態電解質漏液問題。並將之與微小型恆電位儀整合，發展一套可攜式丙酮氣體感測器。此感測器系統可區別糖尿病患者與正常人呼氣成份中之丙酮濃度，並找出糖尿病患者呼氣丙酮濃度與血糖濃度之間的關係，即時監控糖尿病患者的病情。
本研究以電鍍鉛為工作電極、改良式銀/氯化銀為參考電極、網印金為輔助電極，含有離子液體BMIPF6之PVdF(HFP)高分子當作固態電解質組合而成。於室溫下，對此感測器施加電壓-1.1V(vs. modified Ag/AgCl)進行感測，對丙酮氣體之感測靈敏度為0.52μAcm-2ppm-1，偵測極限可達1ppm。在工作電極對感測結果的探討中，發現電鍍鉛是感測丙酮氣體最佳電極，電鍍條件中，電鍍溫度及電流密度皆為影響感測的因素；而在高分子電解質薄膜對感測的結果探討，奈米級二氧化矽為添加物時其靈敏度最高，添加量為20wt%有最大之感測電流；其他感測特性方面，發現相對濕度提高至58及100%時，雖會使電流密度增大，但雜訊增加且靈敏度下降。感測器之選擇性測試方面，二氧化碳、氨氣及異戊二烯氣體對丙酮感測並不會造成干擾；與微小型恆電位儀整合後，此感測系統所得之靈敏度0.58μAcm-2ppm-1，且與商用恆電位儀所測得之電流相關係數高達0.997；臨床應用上，不管是商用或微小型恆電位儀感測器系統皆可明確的將患者及健康者作一區別。並利用商用及微小型恆電位儀測量糖尿病患者之呼氣丙酮值，發現丙酮氣體與血糖之相關係數為0.501及0.521，顯示糖尿病患者中之呼氣丙酮值與血糖確實呈現正相關，使此套可攜式丙酮感測器系統對於應用於糖尿病診斷更具有潛力。

According to medical reports, diabetes have higher acetone concentration in their expired air; thus, the determination of acetone in expired air is useful for diagnosis of diabetes. It would be possible to substitute traditional blood test method. The screen-printing and electroplating method were used to prepare working electrode. And the solid polymer electrolyte could prevent leakage. It developed one portable acetone sensing system by integrating home-made potentiostat. By using the sensing system, the difference of acetone responses between diabetic patients and healthy person were obtained. The possibility exists to use an acetone sensor system as a means of monitoring the diabetic patient’s condition.

In this study, the sensing system included electroplating lead working electrode, modified Ag/AgCl reference electrode, screen-printing gold counter electrode and solid polymer electrolyte PVdF(HFP) with ionic liquid BMIPF6. At applied potential -1.1V vs. modified Ag/AgCl, the sensitivity of sensing system was 0.52μAcm-2ppm-1, and the detection limit attained to 1ppm. The effects of working electrode materials on sensing, electroplating lead was found to be the best one. It prepared under 25℃ and 40mA/cm2. The solid polymer electrolyte with 20% nano SiO2 were the best preparing conditions. Furthermore, the sensor selectivity was tested. It indicated that carbon dioxide, ammonia and isoprene did not affect the acetone detection. The results of integrating home-made mini portable potentiostat show the good sensitivity to 0.58μAcm-2ppm-1, and the correlation coefficient of measured current between commercial and home-made potentiostat was 0.997. In clinical application, the sensor could clearly distinguish the normal expired air from the diabetic ones. We also measured the expired air of diabetic patients to find out the relationship between concentration of blood sugar and acetone using commercial and home-made potentiostat. The correlation coefficient was 0.501 and 0.521. These values showed that the relationship between blood sugar and concentration of acetone was correlative. The developed acetone sensing system is potential for diagnosis of diabetes.

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