基因表達的轉譯調控機制可分為兩種類型：cap-dependent和cap-independent。IRES (Internal Ribosome Entry Site)位於5'UTR區域，是一個可以吸引ribosome複合物合成蛋白質的調控元件。IRES參與了細胞凋亡和生長因子調節等重要的生理程序且多屬於癌基因。IREZone (Internal Ribosome Entry Zone)為多個IRES結構不間斷且重疊的區域，已被證明具備更強的轉譯調控能力。因此了解基因是否帶有IREZone或IRES對於研究該基因的轉譯調控機制是非常有益的。在本研究中，我們使用scan for matches軟體，以預測每個mRNA 5'UTR transcript是否帶有IRES，然後，我們定義三個重疊以上的IRES區域稱為IREZone。uORF(upstream open reading frames)位於5'UTR的上游轉譯調控元件，多屬於致癌基因且參與重要生理過程的調控。ribosome profiling是提供了基因體在蛋白質合成的資訊的資料，結合ribosome profiling可以清楚看到轉譯與調控元件之間的關係。最後，建立了一個mRNA 5’UTR translational regulation motifs資料庫，其網址為http://cosbi2.ee.ncku.edu.tw/transbrowser/。

The translational regulation mechanisms of gene expression can be divided into two types: Cap-dependent and IRES-dependent. (Internal Ribosome Entry Site) IRES, located in the 5’UTR region, is a cis-acting element which can attract the ribosome complexes to synthesize proteins. IRES is known to be involved in cell apoptosis and the regulation of oncogenes and growth factors. IREZone (Internal Ribosome Entry Zone), a region of multiple overlapping IRES structures, has been shown to have strong translational regulation capability. Therefore, knowing the information of IREZone or IRES in the 5’UTR of a gene is very helpful for studying the translational regulation mechanism of that gene. In this study, we use the Scan-For-Matches software to identify the IRES in the 5’UTR region of each human mRNA transcript. Then we define IREZone as a region with at least three overlapping IRES. uORF is the open reading frames locates 5’UTR upstream and most of them which involve in some important regulations of biological process belong to Oncogene. Ribosome profiling provides information of genetic synthesis in proteins. It can understand clearly relations between translational control and cis-acting element by means of combining analysis of ribosome profiling. Finally, we construct a database called mRNA 5’UTR translational regulation motifs, that is available online at http://cosbi2.ee.ncku.edu.tw/transbrowser/.

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