最近幾年癌症已變成最危險的疾病之一，而癌症最典型的治療方式是化療以及放射性治療，而這些伴隨著許多副作用。而近幾年許多研究人員投入電磁場與生物相互關係的研究裡，無論是從正常的人類細胞甚至到癌細胞都對電磁場有不同程度上的反應,許多研究顯示癌症細胞對於極低頻電磁場具有高度的敏感性，在電磁場的刺激下癌症細胞會有抑制現象的發生，而對正常細胞無抑制。此外在相關論文提到電磁場影響鈣離子濃度，進而影響惡性細胞生長。基於以上的理由，我們嘗試利用舒曼共振頻率7.83 Hz結合電磁場來探討其對不同期乳細胞的影響性，並進一步使用不同頻率的方式來測試。
我們的研究結果顯示，惡性細胞在舒曼共振頻率7.83 Hz電磁場刺激之下有明顯的抑制現象，而正常細胞無抑制的現象。而癌細胞的存活率隨著頻率提高也有所變化，而我們推論此結果可能與鈣離子共振現象有關，進一步影響到癌細胞正常的生長。

In recent years, cancer has become one of the most dangerous diseases, the most typical treatments of cancer are chemotherapy and radiation therapy, but these methods are accompanied by many side effects. Recently, many researchers have been involved in the study of the relationship between electromagnetic fields and biology. Both normal human and cancer cells have different degrees of response to electromagnetic field. Many studies have shown that cancer cells are high sensitivity of extremely low frequency electromagnetic field. In the stimulation of the electromagnetic field cancer cells will be inhibited, and no inhibition of normal cells. Moreover, the relevant papers mentioned that the electromagnetic field affects the calcium ion concentration, while affecting the growth of malignant cells. Base on above reasons, we try to use 7.83 Hz which is called Schumann resonance frequency, combining with low magnetic field intensity to influence different stage of breast cells, and further use different frequencies to test.
Our results show that the malignant cells exposed to the　Schumann resonance of 7.83Hz electromagnetic field are significantly inhibited, in contrast, the normal cells are not inhibited. The cell viability rate of cancer cells also changes with the frequency, and we infer that this result may be related to the phenomenon of calcium ion resonance, and further affect the normal growth of cancer cells.

[1] Belova, Natalia A., and Daniel Acosta-Avalos. "The Effect of Extremely Low Frequency Alternating Magnetic Field on the Behavior of Animals in the Presence of the Geomagnetic Field." Journal of Biophysics, 2015.

[2] Lacy-Hulbert, A., J.C. Metcalfe, and R. Hesketh, Biological Responses to Electromagnetic Fields. FASEB Journal: Official Publication Of The Federation Of American Societies For Experimental Biology, vol. 12(6), pp. 395-420, 1998.

[3] Brighton CT, Wang W, Seldes R, Zhang G, Pollack SR. "Signal Transduction in Electrically Stimulated Bone Cells," The Journal of Bone & Joint Surgery, vol. 83, pp. 1514-1523, 2003.

[4] Christina L. Ross, Mevan Siriwardane, Graça Almeida-Porada, Christopher D. Porada, Peter Brink, George J. Christ, Benjamin S. Harrison, “The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation,” Stem Cell Research, vol. 15, pp. 96-108, 2015.

[5] Tingting Wang,Yunzhong Nie,Shuli Zhao,Yuwang Han, Youwei Du, and Yayi Hou, “Involvement of Midkine Expression in the Inhibitory Effectsof Low-Frequency Magnetic Fieldson Cancer Cells, “Bioelectromagnetics, vol. 32, pp. 443-452, 2011.

[6] Yunzhong Nie, Leilei Du, Yongbin Mou, Zhenjun Xu, Leihua Weng, Youwei Du, Yanan Zhu, Yayi Hou and Tingting Wang, “Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation,” BMC Cancer, vol. 13, pp. 582, 2013.

[7] Carly A. Buckner, Alison L. Buckner, Stan A. Koren, Michael A. Persinger, Robert M. Lafrenie, “Inhibition of Cancer Cell Growth by Exposure to a Specific Time-Varying Electromagnetic Field Involves T-Type Calcium Channels,” PLoS ONE, vol. 10, 2015.
[8] JW Zimmerman, MJ Pennison, I Brezovich2, N Yi, CT Yang, R Ramaker, D Absher, RM Myers, N Kuster, FP Costa, A Barbault and B Pasche. “Cancer cell proliferation is inhibited by specific modulation frequencies,” British Journal of Cancer, vol. 106, pp. 307-313, 2012.

[9] S. J. Palmer A M. J. Rycroft A M. Cermack. “Solar and geomagnetic activity, extremely low frequency magnetic and electric fields and human health at the Earth’s surface,” Surv Geophys, vol. 27, pp. 557–595, 2006.

[10] Neil Cherry. “Schumann Resonances, a plausible biophysical mechanism for the human health effects of Solar/Geomagnetic Activity.” Natural Hazards, vol. 26, pp. 279–331, 2002

[11] N. J. Cherry. “Human intelligence: The brain, an electromagnetic system synchronised by the Schumann Resonance signal,” Medical Hypotheses, vol. 60, pp. 843–844, 2003.

[12] Irena cosic, Dean Cvetkovic, Qiang Fang, Emil Jovanov, Harry Lazoura. “Human Electrophysiological Signal Responses to ELF Schumann Resonance and Artificial Electromagnetic Fields,” FME Transactions, vol. 34, pp. 93-103, 2006.

[13] Ross, C. "Mechanisms of extra low frequency electromagnetic field (ELF-EMF) on human bone marrow stem/stromal cell (hHM-MSC) differentiation." JSM Biotech BME 3, vol. 33, pp. 5-16, 2016.

[14] Zwingelberg R, Rosenthal M, Mevissen M, Buntenkötter S, Löscher W, Obe G. Exposure of rats to a 50-Hz, 30-mT magnetic field influences neither the frequencies of sister-chromatid exchanges nor proliferation characteristics of cultured peripheral lymphocytes. Mutation Research Letters,vol.302(1),pp.39-44. 1993

[15] Ross, Christina L. "The use of electric, magnetic, and electromagnetic field for directed cell migration and adhesion in regenerative medicine." Biotechnology progress, vol. 33.1, pp .5-16. 2017.

[16] Dimitris J. Panagopoulos,a, Andreas Karabarbounis,b and Lukas H. Margaritis. “Mechanism for action of electromagnetic fields on cells," Biochemical and Biophysical Research Communications, vol. 298, pp. 95–102, 2002.
[17] Roderick HL, Cook SJ, “Ca2+ signalling checkpoints in cancer: remodelling Ca2+ for cancer cell proliferation and survival,” Nat Rev Cancer, vol. 8, pp. 361-375, 2008.

[18] Giuliani, Enrico D'Emilia & Settimio Grimaldi (2008) “Calcium Ion Cyclotron Resonance (ICR) Transfers Information to Living Systems: Effects on Human Epithelial Cell Differentiation, Electromagnetic Biology and Medicine, vol. 27:3, pp. 230-240, 2008.

[19] Carly A. Buckner, Alison L. Buckner, Stan A. Koren, Michael A. Persinger, Robert M. Lafrenie, “Inhibition of Cancer Cell Growth by Exposure to a Specific Time-Varying Electromagnetic Field Involves T-Type Calcium Channels,” PLoS ONE, vol. 10, 2015.

[20] Lyle, D.B., et al., Calcium-uptake by leukemic and normal T-lymphocytes exposed to low-frequency magnetic-fields. Bioelectromagnetics, vol.12, pp.145-156, 1991.

[21] Ewa Lindstrom, Per Lindstrom, Andre Berglund, Erik Lundgren, and Kjell Hansson Mild, lntracellular Calcium Oscillations in a T-cell Line After Exposure to Extremely- Low-Frequency Magnetic Fields With Variable Frequencies and Flux Densities, Bioelectromagnetics, vol.16, pp.41-47, 1995.

[22] Lisi, A., et al., Ion Cyclotron Resonance as a Tool in Regenerative Medicine. Electromagnetic Biology & Medicine, vol.27, pp. 127-133. 2008.

[23] Foletti, A., et al., Cellular ELF Signals as a Possible Tool in Informative Medicine. Electromagnetic Biology & Medicine, vol.28, pp. 71-79. 2009.

[24] Lisi, A., et al., Calcium Ion Cyclotron Resonance (ICR) Transfers Information to Living Systems: Effects on Human Epithelial Cell Differentiation. Electromagnetic Biology & Medicine, vol. 27, pp. 230-240. 2008.

[25] Foletti, A., et al., Calcium Ion Cyclotron Resonance (ICR), 7.0 Hz, 9.2 μT Magnetic Field Exposure Initiates Differentiation of Pituitary Corticotrope-Derived AtT20 D16V Cells. Electromagnetic Biology & Medicine, vol. 29(3), pp. 63-71. 2010.