奈米塑膠（Nanoplastics, NPs）被定義為粒徑小於1 μm的塑膠顆粒，因其具有生物累積潛力及相關的生態與健康風險，已成為日益受到關注的環境污染物。然而，在複雜的生物基質中偵測與定量NPs仍是一項重大分析挑戰，阻礙了對其環境生物影響的準確評估。
本研究開發了一種基於顆粒數濃度的方法，將奈米塑膠與金奈米顆粒標記後，利用單顆粒感應耦合電漿質譜進行偵測。此方法應用於評估天然有機質（Natural organic matter, NOM）對斑馬魚體內奈米塑膠生物累積的影響。我們針對兩種代表性 NPs: 70 nm 聚苯乙烯與105 nm 聚甲基丙烯酸甲酯在有無天然有機物存在下的生物累積行為進行探討。
結果顯示，天然有機物顯著改變了奈米塑膠的攝取模式，增加了其在鰓、肝臟與腸道中的累積，卻降低了在肌肉中的累積。這種分布模式可能歸因於天然有機物改變了奈米塑膠的表面性質，促進其在高代謝活性器官中的吸收，同時減少在肌肉中的累積。
這些結果強調，在評估奈米塑膠的生物累積與環境風險時，需將天然有機物的影響納入考量，以確保風險評估的準確性。

Nanoplastics (NPs), defined as plastic particles smaller than 1 μm, have emerged as environmental contaminants of increasing concern due to their potential for bioaccumulation and associated ecological and health risks. However, detecting and quantifying NPs in complex biological matrices remains a significant analytical challenge, hindering accurate evaluation of their environmental fate and biological impacts.
In this study, we developed a particle number concentration-based method by labeling NPs with gold nanoparticles (AuNP) and detecting them using single-particle ICP-MS (spICP-MS). This method was applied to evaluate the influence of natural organic matter (NOM) on the bioaccumulation of NPs in zebrafish. We examined the bioaccumulation behavior of two representative NPs: 70 nm polystyrene (PS) and 105 nm poly methyl methacrylate (PMMA) in the presence and absence of NOM. The results revealed that NOM significantly modified NPs uptake, increasing accumulation in the gills, liver, and intestine while reducing it in muscle tissue. This pattern may be attributed to NOM altering the surface properties of NPs, which promotes their uptake in highly active organs while reducing accumulation in muscle. These results underscore the importance of considering NOM when assessing nanoplastic bioaccumulation, which is essential for accurate environmental risk evaluation.

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