最近幾年生物科技在細胞方面的研究都趨向於使用三維立體環境來培養以及試驗細胞特性，這是因為細胞生長在體內的環境是擁有三維的支架。傳統的二維培養往往無法切確模擬出細胞在體內的生長狀況，使得細胞的生物物理和生物化學受到限制，這些限制往往會影響藥物試驗、再生醫學和基礎科學的研究，進而導致實驗上的精度及可靠度降低。在大多數的研究中都習慣使用合成的水凝膠(膠原凝膠)混合培養液來創造立體支架，以達到立體培養的目的，但大多數三維細胞培養往往都只侷限在影像的觀察及生化試劑的量測，對於細胞在三維培養環境下的電特性的數據十分的匱乏。細胞電特性，如細胞膜電容與細胞質電阻，可提供許多訊息來研究細胞膜與細胞質所發生的變化，且不需複雜的生化檢測。
細胞阻抗分析被廣泛地應用於監測生物與藥物反應，而本研究利用微影、電鑄技術製備了結合三維電極的三維培養環境，用以培養以及電阻抗量測細胞在三維培養環境下的電特性，在經過十二小時左右的細胞培養，黑色素腫瘤癌細胞也順利地進行分裂，而分裂的電阻抗數據，也將應用在細胞等校電路的探討。

In recent years, the biological technology of cell culture tends to use three-dimensional environment to culture and test cell properties, because the cells have a three-dimensional scaffold in vivo growth environment. The traditional two-dimensional culture environment which can’t correctly simulate the vivo growth conditions for cells, especially the biophysics and biochemistry are restricted by the traditional two-dimensional culture environment. These restrictions tend to affect the accuracy and the reliability for the drug trial, regenerative medicine, and the research of basic science. In the most researches, they were accustomed to using the synthetic hydrogels (collagen gel) which mixes with medium to create three-dimensional scaffold, that can reach the purpose of three-dimension (3D) cell culture. But the most of the biological technology usually restricted in the image observation and measurement of biochemical reagents, the electrical properties of cell culture in three-dimensional environment were very scarce. The membrane capacitance (Cc) and cytoplasm resistance (Rc) can provide the information required to investigate changes in the membrane and cytoplasm without the need for complex chemical biochemical detection.
Cell impedance analysis is widely used for monitoring biological and medical reactions. In this study, the three-dimenional (3D) microelectrodes combined with the three-dimensional (3D) culture environment were fabricated for Electrical Properties of B_16 F_10 using electroforming and lithography technology. After about twelve hours cultured, the B_16 F_10 cancer cells successfully divided in three-dimensional (3D) microelectrodes. The data of cell-division were explored in the equivalent circuit of the cell.

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