添加有機鹼於酵素催化之反應中具有提高選擇性及增加反應性的優點，而其反應機制至今尚未明確證實。

本論文旨在研究反應溶劑中添加有機鹼的作用，藉以提高木瓜脂肪分解酵素對外消旋naproxen三氟乙硫酯之水解分割反應速率，使得木瓜脂肪分解酵素在硫酯系統中除了可以維持良好的選擇性外，更可提高反應性，以符合工程需要。

論文中嘗試添加三乙基胺、1-methylpiperdine (MP)、三辛基胺等在水溶液中pKa値較高的鹼，以及含氮結構的吡啶與4-dimethylaminopyridine (DMAP)。實驗結果顯示以添加30 mM三辛基胺於系統之反應性提高為1.5倍最佳，接著是添加MP、三乙基胺及吡啶等皆有酵素活化的現象；DMAP則會對酵素產生嚴重抑制，添加4 mM即會造成反應性下降一半。

本論文以基質為naproxen三氟乙酯的反應系統所發展之模式為根據，加以修正後嘗試提出與研究結果相符之理論模式，並試著求出內含之動力學參數。偶合結果顯示所推論的反應機構模式未能完整描述本系統之行為，表示理論模式尚有改進的空間。

Addition of various organic bases to the reaction medium for the enzyme-catalyzed kinetic resolution can enhance the enzyme enantioselectivity and activity; yet there is no exact mechanism proposed for elucidating this effect till now. In this study, we try to find out the base additives effect on papaya lipase-catalyzed hydrolytic resolution of (R,S)-naproxen 2,2,2-trifluoroethyl thioester.

The bases employed in this study include triethylamine, 1-methylpiperdine (MP), and trioctylamine of higher pKa values, as well as pyridine and 4-dimethylaminopyridine (DMAP) containing a pyridine moiety. Trioctylamine is selected as the best enzyme activator, as the initial rate for the (S)-enantiomer increases about 1.5-fold. Moreover, MP, triethylamine and pyridine have less improvement on the lipase performance. On the other hand, DMAP acts as the lipase inhibitor, and half of the enzyme activity remain when adding 4 mM of the base.

By modifying the kinetic model previously proposed for the lipase-catalyzed hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester, we try to estimate the kinetic constants by adding various bases. Comparisons and elucidations of the experimental data with the theoretical results are also made.

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