| 研究生: |
林政弘 Lin, Cheng-Hung |
|---|---|
| 論文名稱: |
居家健檢系統開發應用於糞便潛血量測之研究 Development of Home Health Checkup System for Detecting Fecal Occult Blood |
| 指導教授: |
林裕城
Lin, Yu-Cheng |
| 學位類別: |
碩士 Master |
| 系所名稱: |
工學院 - 工程科學系 Department of Engineering Science |
| 論文出版年: | 2019 |
| 畢業學年度: | 107 |
| 語文別: | 中文 |
| 論文頁數: | 115 |
| 中文關鍵詞: | 糞便潛血 、影像處理 、行動醫療應用軟體 、統一計算架構 、HSV色彩空間 |
| 外文關鍵詞: | home health checkup, fecal occult blood, image processing, mobile medical application, CUDA, HSV |
| 相關次數: | 點閱:107 下載:0 |
| 分享至: |
| 查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
本研究成功開發出具有線性度、穩定度之可於開放環境中使用的居家健檢系統,利用免疫反應呈色作為檢測標的並以本系統量化其結果。本研究中使用所開發之應用程式與伺服器連接、拍攝免疫反應呈色結果與本研究所開發的比色板並上傳至伺服器以達到雲端運算的功能,並接收運算結果存入應用程式中的資料庫記錄日期與濃度,以供使用者未來瀏覽使用。為確認本研究所開發的居家健檢系統能於不同偏光環境、對焦距離、光強度、不同手機下有相似的線性度,本研究同時開發一可量化環境的模擬環境系統作為輔助系統以供測試居家健檢系統時使用。
本研究中的居家健檢系統其應用程式使用於Android作業系統以Android Studio IDE使用Java程式語言開發,使用的網頁伺服器為XAMPP,並以PHP做資料傳輸與管理。伺服器中的影像處理程式以Visual Studio IDE做為開發環境,整合HSV色彩空間之概念、OpenCV開源程式庫與Nvidia所開發的CUDA架構以C/C++撰寫。
在模擬環境系統中的測試,本研究所開發的居家健檢系統其影像處理程式於開放環境下仍可正確的抑制陰影、環境光與人為操作上的誤差並且正確的得到T線特徵位置。並於偏光、對焦高度與光強度等不同環境變數下其灰階靈敏度測試的曲線決定係數可介於0.978至0.999之間,而於實際糞便潛血檢測劑量量測下其曲線決定係數可達0.956,且可良好的分辨0 ng/mL與50 ng/mL。
This thesis presents a health care system with fast, portability, stability, and low cost for fecal occult blood tests (FOB) to replace the traditional health checkup. The inspection is based on immunochromatographic assay which is widely applied to many fields of clinical diagnosis. In combination with immunochromatographic strip and home health checkup System, the patient is able to detect and calculate the concentration of the sample. Furthermore, the patient can browse the diagnostic records, view the trend and have a health checkup in any place by the application. The system software integrate HSV image processing, cloud computing, Compute Unified Device Architecture (CUDA) acceleration, Android application and server management. Compare with traditional health checkup, to operate the home health checkup system, the only additional hardware is a stage with color palette made by cardboard and the user’s mobile device. To confirm the system with confirmation experiments, we also build a support system which is use to simulate the difference environment. After the test, we found that the home health checkup system is strong enough to operate correctly in most of the condition in daily routine and it is able to present an agile, high sensitive and steady performance with low economical cost.
[1]R. B. Carlsen, O. P. Bahl and N. Swawinathan, “Human Chorionic Gonadotropin Linear Amino Acid Sequence of The β Subunit,” Journal of Biological Chemistry, 248, pp. 6810-6827, 1973.
[2]R. B. Carlsen, O. P. Bahl and N. Swawinathan, “Human Chorionic Gonadotropin Linear Amino Acid Sequence of The β Subunit,” Journal of Biological Chemistry, 248, pp. 6810-6827, 1973.
[3]R. H. Garrett and C. M. Grisham, Biochemistry, Saunders College Publishing, 1995.
[4]E. Engvall and P. Perlmann, “Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme -labeled anti-immunoglobulin in antigen-coated tubes,” Journal of Immunology, 109, pp. 129-135, 1972.
[5]T. Watanabe, Y. Ohkuno, H. Matsuoka, H. Kimura, Y. Sakai, Y. Ohkaru, T. Tanaka, and Y. Kitaura, “Development of a simple whole blood panel test for detection of human heart –type fatty acid-binding protein,” Clinical Biochemistry, 34, pp. 257-263, 2001.
[6]M. Hedenfalk, P. Adlercreutz, and B. Mattiasson, “Modulation of the measuring range of a radioimmunoassay using an organic water two phase system,” Analytica Chimica Acta, 341, pp. 269-274, 1997.
[7]F. Hardy, L. Djavadi-Ohaniance and M. E. Goldberg, “Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assay,” Journal of immunological methods, 200, pp. 155-159, 1997.
[8]P. Onnerfjord, S. Eremin, J. Emneus and G. Marko-Varga, “Fluorescence polarisation for immunoreagent characterization,” Journal of immunological methods, 213, pp. 31-39, 1998.
[9]J. A. Schmid and A. Billich, “Simple method for high sensitivity chemiluminescence ELISA using conventional laboratory equipment,” BioTechniques, 22, pp. 278, 1997.
[10]C. A. Janeway, P. Travers, M. Walport, and M. J. Shlomchik, Immunobiology, 5th edition, Garland Science, 2001.
[11]徐敘瑢,陳憲偉,光電材料與顯示技術,五南文化事業出版,2007.
[12]A.M. Shabut, M.H. Tania, K.T. Lwin, B.A. Evans, N.A. Yusof, K.J. Abu-Hassan, M. Hossain, “An intelligent mobile-enabled expert system for tuberculosis disease diagnosis in real time,” Expert Systems with Applications, 114, pp. 65-77, 2018.
[13]J.Y. Huang, H.T. Lin, T.H. Chen, C.A. Chen, H.T. Chang, C.F. ChenSignal, “amplified gold nanoparticles for cancer diagnosis on paper-based analytical devices, 98,” ACS Sensors, pp. 174-182; 2018.
[14]G. Chen, W. Chen, Y. Yen, C. Wang, H. Chang, C. Chen “Detection of mercury(II) ions using colorimetric gold nanoparticles on paper-based analytical devices, 86,” ACS Publication, pp. 6843-6849, 2014.
[15]Y. Lu, Weiwei Shi, Jianhua Qin, Bingcheng Lin, “Low cost, portable detection of gold nanoparticle‐labeled microfluidic immunoassay with camera cell phone,” Journal of Electrophoresis, 30(4) ,pp. 579 -582, 2009.
[16]R. Cucchiara, C. Grana, M. Piccardi, A. Prati, and S. Sirotti, “Improving shadow suppression in moving object detection with hsv color information,” Intelligent Transportation Systems (August 2001), pp. 334-339.
[17]N. Herodotou, K.N. Plataniotis, A.N. Venetsanopoulos, “A color segmentation scheme for object-based video coding,” Proceedings of the IEEE Symposium on Advances in Digital Filtering and Signal Processing, pp. 25-29, 1998.
[18]H. K, Yuen and Princen, J. and Illingworth, J. and Kittler, J., “Comparative study of Hough transform methods for circle finding. Image Vision Comput,” 81, pp 71–77, 1990 .
[19]J. Canny, “A Computational Approach to Edge Detection,” IEEE Trans. on Pattern Analysis and Machine Intelligence, 8(6), pp. 679-698, 1986.
[20]N. Otsu, “A threshold selection method from gray-level histograms,” IEEE Trans. On Systems, Man, and Cybernetics, SMC-9, pp. 62-66, 1979.
[21]CUDA parallel computing, http://docs.nvidia.com/cuda/parallel-thread-execution/index.html