由於松針落葉的自然分解需要消耗更多時間，導致針葉林中大量的松針落葉長時間堆積，容易引發災害造成巨大損失。本研究基於此，意在更好瞭解松針落葉分解情況，使災害可能變小。臺灣二葉松（Pinus taiwanensis）與臺灣五葉松（Pinus morrisonicola）是臺灣特有種，生長良好且具有代表性，作為取樣對象十分合適。為了更好瞭解本地植物種的落葉分解情況，避免可能的災害，需要更多瞭解松針分解微生物。盡可能多的篩選出分解者，進行觀察和研究。本實驗的目的是從臺灣二葉松及臺灣五葉松松針落葉中篩選出松針分解微生物，對其進行觀察、分類及進一步研究。本研究發現，在松針分解微生物的篩選過程中，相比於其他影響因素，枯枝落葉層與發酵層中出現的微生物種類差別更大，可以看出在分解篩選過程中表面有機層的影響尤其重大。其中有六種微生物更常出現在發酵層中或於發酵層中長勢更加，而枯枝落葉層則只有一種，發酵層相比枯枝落葉層能篩選出更多的微生物。本研究同時依形態區分共篩選出了 13 個類型的松針分解微生物。在對其進行分解能力的測試後，發現Aspergillus屬的3種真菌在纖維素培養基上初始生長速度更快、長勢更好、持續時間更久，推測其最具分解松針中纖維素之能力。

The natural decomposition of pine needle litter takes more time, resulting in the accumulation of large amounts of pine needle litter in coniferous forests over a long period of time, which can lead to catastrophes and significant losses. This study was conducted to better understand the decomposition of pine needle litter and to minimize the potential for disaster. Pinus taiwanensis and Pinus morrisonicola are endemic to Taiwan, and are well established and representative, so they are suitable for sampling. In order to better understand the decomposition of needle litter of local plant species and avoid possible disasters, we need to know more about the decomposing microorganisms of pine needles. As many decomposers as possible can be selected for observation and research. The purpose of this experiment was to screen out pine needle decomposing microorganisms from the needle litter of Pinus taiwanensis and Pinus morrisonicola in Taiwan, and to observe, classify, and further study them. In this study, we found that in the screening process of pine needle decomposition microorganisms, the differences between the litter layer and the fermentation layer were greater than other influencing factors, and the influence of the LFH layer was particularly significant in the screening process. Six microorganisms were more frequently found in the fermentation layer or grew more strongly in the fermentation layer, while only one was found in the litter layer, and more microorganisms were screened in the fermentation layer than in the litter layer. In this study, 13 types of pine needle decomposing microorganisms were selected by morphology. After testing their decomposition ability, three species of Aspergillus were found to have faster initial growth rate, better growth and longer duration on the cellulose medium, and were presumed to be the most capable of decomposing cellulose in pine needles.

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