記憶在穩固化和再穩固化的階段，對於失憶及提升記憶的治療是相當容易受影響的。目前已知電痙攣刺激會減損記憶的穩固化作用。因此，我們假設記憶的再穩固化作用也可以受電痙攣刺激干擾。由古柯鹼誘發的小鼠制約場地偏好學習記憶模式中，發現當提取制約場地偏好的記憶後，立即在小鼠的兩耳耳廓上施予200毫秒的電痙攣刺激(500V, 25mA)，該學習記憶的後來表現會因此減弱。相對的，未經提取制約場地偏好記憶時，雖然施予相同的電痙攣刺激，對場地偏好的行為表現並無影響。當記憶被提取後立即給予全身性Pentobarbital麻醉注射，並不影響爾後的制約場地偏好學習記憶表現；該現象在不同的電痙攣刺激程序，都發現Pentobarbital麻醉下的電痙攣刺激，對爾後的記憶表現並無影響。最後，分別以食物誘發的制約場地偏好學習記憶模式及被動規避學習試驗(Passive avoidance learning test)，發現在非麻醉狀況下的動物，提取相關記憶後，立即給予電痙攣刺激，同樣可以減低動物對食物求償及恐懼記憶的行為表現。綜合以上結果，經由Pavlovian條件所建立的相關記憶，藉由短暫性的電痙攣刺激，可以破壞該記憶的再穩固化作用，降低爾後的行為表現，即使這些記憶已經形成或穩固一段時間，仍然有效。

The deduced memory consolidation and reconsolidation phases are both sensitive to similar amnesic and memory-enhancing treatment. Electroconvulsive stimuli have been well known to impair memory consolidation. Therefore, we hypothesized that electroconvulsive stimuli may disrupt memory reconsolidation. Delivery of a 200-msec electroconvulsive stimulus (500V, 25mA) between mouse pinnas immediately after recall of the cocaine-induced conditioned place preference (CPP) impaired the reconsolidation and subsequent expression of this memory. In contrast, a similar electroconvulsive stimulus did not affect subsequent cocaine-induced CPP performance when the recall procedure was omitted. Systemic pentobarbital injection immediately after the CPP test did not affect subsequent cocaine-induced CPP. Interestingly, various electroconvulsive procedures given immediately after the recall of cocaine-induced CPP did not affect subsequent CPP performance when mice were anesthetized by pentobarbital during the electroconvulsive procedures. Finally, delivery of this 200-ms electroconvulsive stimulus immediately after the reactivation of passive avoidance (PA) and food-induced CPP impaired subsequent performance of these memories in the retest. Taken together, we conclude that a short-lasting electroconvulsive stimulus can deteriorate the reconsolidation of various Pavlovian associative memories, therefore impair their subsequent performance even these memories are already established and stabilized.

1.Abel, T. and Lattal, K.M. Molecular mechanisms of memory acquisition, consolidation and retrieval. Curr. Opin. Neurobiol. 11:180-187, 2001.
2.Alberini, C.M. Mechanisms of memory stabilization: are consolidation and reconsolidation similar or distinct processes? Trends Neurosci. 28:51-56. 2005.
3.Brewin, C.R. The nature and significance of memory disturbance in posttraumatic stress disorder. Ann. Rev. Clin. Psychol. 7:203-207, 2011.
4.Cherng, C.G. and Yu, L. Disruption of conditioned drug memories. Chin. J. Physiol. 52:264-272, 2009.
5.Davis, S. Renaudineau, S., Pririer, R., Poucet, B., Save, E. and Laroche, S. The formation and stability of recognition memory: what happens upon recall? Front. Behav. Neurosci. 4:177, 2010.
6.Dudai, Y. Molecular bases of long-term memories: a question of persistence. Curr. Opin. Neurobiol. 12:211-216, 2002.
7.Dudai, Y. and Eisenberg, M. Rites of passage of the engram: reconsolidation and the lingering consolidation hypothesis. Neuron 44:93-100, 2004.
8.Fan, H-Y., Cherng, C.G., Yang, F-Y., Cheng, L-Y., Tsai, C-Y., Lin, L-C. and Yu, L. Systemic treatment with protein synthesis inhibitors attenuates the expression of cocaine memory. Behav. Brain Res. 208:522-527, 2010.
9.Ho, M-C., Cherng, C. G., Tsai, Y-PN., Chiang, C-Y., Chuang, J-Y., Kao, S-F. and Yu, L. Chronic treatment with monoamine oxidase-B inhibitors decreases cocaine reward in mice. Psychopharm. 205:141-149, 2009.
10.Hupbach, A., Gomez, R., Hardt, O. and Nadel, L. Reconsolidation of episodic memories: a subtle reminder triggers integration of new information. Learn. Mem. 14:47-53, 2007.
11.Lin, K-Y., Cherng, C.G., Yang, F-Y., Lin, L-C., Lu, R-B. and Yu, L. Memantine abolishes the formation of cocaine-induced conditioned place preference possibly via its IL-6-modulating effect in medial prefrontal cortex. Behav. Brain Res. 220: 126-131, 2011.
12.Meeter, M., Murre, J.M., Janssen, S.M., Birkenhager, T. and van den Broek, W.W. Retrograde amnesia after electroconvulsive theraph: A temporary effect? J. Affect. Disord. 132:216-222, 2011.
13.Nader, K. and Hardt, O. A single standard for memory: the case for reconsolidation Nat. Rev. Neurosci. 10:224-234, 2009.
14.Nader, K., Schafe, G.E. and LeDoux, J.E. The labile nature of consolidation theory. Nat. Rev. Neurosci. 1:216-219, 2000.
15.Nishihara, F. and Saito, S. Pre-ictal bispectral index has a positive correlation with seizure duration electroconvulsive therapy. Anesth. Analg. 94:1249-1252, 2002.
16.Nishihara, F. and Saito, s. Adjustment of anesthesia depth using bispectral index prolongs seizure duration in electroconvulsive therapy. Anaesth. Intensive Care 32:661-665, 2004.
17.Ochiai, R., Yamada, T., Kiyama, S., Nakaoji, T., Takeda, J. Bispectral index as an indicator of seizure inducibility in electroconvulsive therapy under thiopental anesthesia. Anesth. Analg. 98:1030-1035, 2004.
18.O'Connor, M., Lebowitz, B.K., Ly, J., Panizzon, M.S., Elkin-Frankston, S., Dey, S., Bloomingdale, K., Thall, M., Pearlman, C. A dissociation between anterograde and retrograde amnesia after treatment with electroconvulsive therapy: a naturalistic investigation. J. ECT 24:146-151, 2008.
19.Sara, S.J. Retrieval and reconsolidation: toward a neurobiology of remembering. Learn. Mem. 7:73-84, 2000.
20.Semkovska, M., McLoughlin, D.M. Objective cognitive performance associated with electroconvulsive therapy for depression: a systematic review and meta-analysis. Biol. Psychiatry. 68:568-577, 2010.
21.Tronson, N.C., Taylor, J.R. Molecular mechanisms of memory reconsolidation. Nat. Rev. Neurosci. 8:262-275, 2007.
22.Von der Goltz, C., Kiefer, F. Learning and memory in the aetiopathogenesis of addiction: future implications for therapy? Eur. Arch. Psychiatry. Clin. Neurosci. 259, Suppl 2:S183-187, 2009.
23.White, P.F., Rawal, S., Recart, A., Thornton, L., Litle, M., Stool, L. Can the bispectral index be used to predict seizure time and awakening after electroconvulsive therapy? Anesth. Analg. 96:1636-1639, 2003.