蛋白質微陣列已成為蛋白質體學研究中的重要工具。蛋白質微陣列的優勢在於可同時進行大量分析，但所需成本較高、製作時間較久，故降低成本及縮短製程時間有其必要性。本研究的目的在於整合自組裝單分子層技術與微機電製程，製作一生醫檢測之蛋白質微陣列螢光檢測晶片。本研究主要分成兩個部分，第一部份是使用微機電製程在玻璃基材上製作蛋白質微陣列反應型晶片。另一部分即是微陣列玻璃基材的表面處理及蛋白質固定化技術。APTES (3-氨基丙基三甲氧基甲矽烷) 跟蛋白質A 被用來製作一連串的蛋白質固定化實驗。實驗開始於使用FITC 螢光標定與APTES 互相結合，觀察APTES在玻璃基材上的成膜效果。由於大部分傳統方法皆使用長時間浸泡法製作自組裝單分子層，本研究嘗試經由固定FITC 螢光標定之蛋白質A，改變反應時間跟溫度探討APTES成膜的反應機制。由螢光強度的實驗結果顯示，APTES 於反應時間4 分鐘以及反應溫度25 ℃即可與玻璃基材表面形成共價鍵結反應。另外，固定FITC 螢光標定之antimouse IgG是為了檢測蛋白質A 的活性。更進一步成功的建立了一套牛血清蛋白模組應用於臨床醫學檢測。本研究藉由測量親疏水角量測儀(contact angle goniometry)、原子力顯微鏡(atomic force microscopy, AFM)以及螢光顯微鏡之分析來瞭解基材表面被自組裝單分子層修飾及固定生物分子後的表面特性。此蛋白質微陣列螢光檢測晶片，解決了傳統生醫檢測系統過於龐大、成本昂貴的缺點，以實現Lab on a Chip 的理想。

Silicon has been extensively used as the primary material in the MEMS manufacturing process. However, it is not suitable for biomedical applications because of its long-term bio-toxicity and lack of opacity, which cause adverse effects and impair the analytical accuracy in a biological system. Therefore, there is an emerging need to develop fabrication techniques using proper materials for bio-analysis applications. The goal of the study is to fabricate protein chips by combining the techniques of MEMS and Self-Assemble monolayer. This work presents a fabrication process of protein chips based on glass substrate using APTES and protein A. The immobilization of APTES on glass substrate is demonstrated using fluorescein isothiocyanate (FITC). The effects of reaction temperature and time on silanization of APTES are investigated to elucidate the mechanism of APTES. The normalized fluorescent intensity indicates that a short period (4 min) of silanization at 25 ℃ suffices to form an APTES thin film. Additionally, a subsequent experiment on the immobilization of FITC-labeled antimouse IgG reveals favorable activation of the protein A. Moreover, rabbit anti-bovine albumin–BSA–sheep anti-bovine albumin that is conjugated with FITC, is used to establish a model for clinical medicine applications. The results demonstrate that APTES can be used to fabricate protein chips as a monolayer under silanization conditions of 4 min at 25 ℃.

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