本研究採用波長為355 nm之脈衝雷射，以不同的脈衝頻率(10~70 kHz)及雷射掃描軌跡間距(1~30 um)，對第四級商業純鈦進行表面熔蝕處理，探討脈衝頻率與掃描軌跡間距對鈦金屬表面潤濕性之影響；其中以30 kHz之脈衝頻率與15 µm之掃描軌跡間距的雷射參數具有最佳的表面潤濕性，該表面之接觸角僅有3.63度，為超親水性表面。接續以最佳潤濕性之雷射參數，搭配低、中、高速之雷射掃描速度使產生不同形貌之週期性結構，並與牙科植體最常被採用之噴砂酸蝕表面與未經處理之機械加工表面進行比較，針對其表面形態、表面粗糙度及表面成分進行差異分析，且透過初生幼鼠頭顱骨細胞的培養來評估雷射熔蝕處理、噴砂酸蝕處理及機加工處理表面之生物相容性。結果顯示高表面粗糙度具有較佳的細胞增殖率，在低掃描速度之雷射處理表面有最高的粗糙度(Ra 3.25µm與Sa 3.99µm)，且材料在雷射熔蝕過程中與空氣反應產生氧化鈦及氮化鈦，幫助了細胞生長，因此細胞增殖率最高；此外，中雷射掃描速度之表面粗糙度雖低於與噴砂酸蝕表面，但因為雷射熔蝕表面具有奈米凝固結構，使其細胞增殖率仍高於噴砂酸蝕表面，證明表面若具有奈米結構，將能有效提升細胞之增殖行為。最後植體以低掃描速度與高掃描速度之參數進行表面處理後，經由動物實驗觀察其植體穩定度、組織學、組織計量學及移除扭力狀況來評估骨整合效果，結果顯示低雷射掃描速度熔蝕後的植體有較佳之骨整合效果，證明植體具有高表面粗糙度、奈米結構與含有氧化鈦和氮化鈦化合物時，可有效提升生物相容性與促進骨整合。

In this study, a pulsed laser with a wavelength of 355 nm was used. The laser parameters with optimal wettability and the low-, medium-, and high traveling speed were used to produce periodic structures with different morphologies. The rat calvarial osteoblast cells were cultured on the surface of laser treated, sandblasted and acid etched, and machined to evaluate the biocompatibility. The results show that the high surface roughness has a better cell proliferation rate, and the highest roughness is obtained on the laser treatment surface at a low traveling speed. Finally, the implant is treated with parameters of low traveling speed and high traveling speed. The osseointegration evaluated by animal experiments. The results show that the implants after laser treated at low traveling speed have better implant stability and osseointegration.

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