由於現在人過於忙碌，人們為求新知，常常超時晚睡、長期熬夜，違反了一般人的正常生理時鐘，也就是所謂的生物節律失調，這種現象會引起很多不良的生理反應。相關研究指出，靜坐練習能進入深層的意識狀態，在生理、心理各方面皆有顯著的放鬆效果。
本研究整合了腦電圖(EEG) 、心電圖(ECG)、肌電圖(EMG)。腦電圖(EEG)偵測因生物節律失調的腦波狀態，心電圖能了解自律神經活性的平衡，肌電圖偵測受測者的肩頸肌肉疲勞狀態。實驗利用匹斯堡睡眠品質量表(PSQI)與生活健康量表(SF-36)，篩選出條件符合的受測者，探討靜坐練習對於熬夜所引發的不良生理反應。結果呈現，靜坐過後腦波的Theta、Alpha波增加，顯示有放鬆趨勢且自律神經系統有漸漸平衡，而閉眼休息卻無法完全讓腦波達到放鬆的效果。

Due to the busy life styles,for the sake of knowledge, often overtime, long-term stay up,that breaks the right biological clock for most people,and this is the so-called biorhythms disorders,this phenomenon will lead to many adverse physiological responses.Related research indicates that Meditation Practice to get into the deep state of consciousness in the physical, psychological aspects have a significant effect of relaxation.
In this study, the integration of the electroencephalogram(EEG), electrocardiogram(ECG),and the electromyography(EMG).Electroencepha-
logram(EEG) to detect biorhythms disorder because of the state of the brain waves. electrocardiogram (ECG) can understand the balance of autonomic nervous system activity , measured by electromyography (EMG) to detect the neck and shoulder muscle fatigue.Experimental use of Pittsburgh Sleep Quality Inventory (PSQI) and life Health Questionnaire (SF-36),selected in line with the conditions of the subjects to explore the improvement of the practice of meditation on unhealthy physiological influence caused by staying up. As the result,after Meditation the brain waves of the Theta, Alpha waves increase, the trend shows that relaxing, the autonomic nervous system has the tendency of the balance. But closed-eye rest can not get nice the effect of brain waves to achieve relaxation.

[1] Janet M. Mullington, Monika Haack, Maria Toth,Jorge M. Serrador and Hans K. Meier-Ewert(2009), Cardiovascular, Inflammatory, and Metabolic Consequences of Sleep Deprivation. Progress in Cardiovascular Diseases, Vol. 51, No. 4 (January/February), pp 294-302
[2] Mari Yokoi, Ken Aoki, Yoshihiro Shiomura,Koichi Iwanaga and Tetsuo Katsuura(2003), J Physiol Anthropol Appl Human Sci, 22: 257–263
[3] Mari Yokoi, Ken Aoki, Yoshihiro Shimomura, Koichi Iwanaga and Tetsuo Katsuura, Exposure to Bright Light Modifies HRV Responses to Mental Tasks during Nocturnal Sleep Deprivation, J Physiol Anthropol, 25: 153–161, 2006
[4] Sun Hee Na, Seung-Hyun Jin , Soo Yong Kim , The effects of total sleep deprivation on brain functional organization:Mutual information analysis of waking human EEG,International Journal of Psychophysiology 62 (2006) 238–242
[5] Ning Li, Yan Wang, Mingshi Wang, Haiying Liu, Effects of sleep deprivation on gamma oscillation of waking human EEG. Progress in Natural Science 18 (2008) 1533–1537
[6] Kaushik, R.M., et al., Effects of mental relaxation and slow breathing in essential hypertension. Complementary Therapies in Medicine, 2006. 14(2): p. 120-126.
[7] Steptoe, A. and Y. Sawada, Assessment of Baroreceptor Reflex Function During Mental Stress and Relaxation. Psychophysiology, 1989. 26(2): p. 140-147.
[8] Jacobs, G.D., H. Benson, and R. Friedman, Topographic EEG mapping of the relaxation response. Biofeedback and Self-Regulation, 1996. 21(2): p. 121-129.
[9] Jacobs, G.D. and R. Friedman, EEG spectral analysis of relaxation techniques. Applied Psychophysiology and Biofeedback, 2004. 29(4): p. 245-254.
[10] Knott, V., Bakish, D., Lusk, S., & Barkely, J. (1997). Relaxation-induced EEG alterations in panic disorder patients. Journal of Anxiety Disorders, 11(4), 365-376.
[11] Tetsuya Takahashi Tetsuhito Murataa, Toshihiko Hamadab, Masao Omoria, Hirotaka Kosakaa, Mitsuru Kikuchic, Haruyoshi Yoshidab, Yuji Wadaa. Changes in EEG and autonomic nervous activity during meditation and their association with personality traits,International Journal of Psychophysiology 55 (2005) 199– 207
[12] Autonomic and EEG Patterns during Eyes-Closed Rest andTranscendental Meditation (TM) Practice: The Basis for a Neural Model of TM Practice, Consciousness and Cognition 8, 302–318 (1999)
[13] Hall, M., et al., Acute stress affects heart rate variability during sleep. Psychosomatic Medicine, 2004. 66(1): p. 56-62.
[14] Kimura, K., et al., Temporal variation of acute stress responses in sympathetic nervous and immune systems. Biological Psychology, 2005. 70(2): p. 131-139.
[15] LeBlanc, J., M.B. Ducharme, and M. Thompson, Study on the correlation of the autonomic nervous system responses to a stressor of high discomfort with personality traits. Physiology & Behavior, 2004. 82(4): p. 647-652.
[16] Reims, H.M., et al., Blood viscosity: Effects of mental stress and relations to autonomic nervous system function and insulin sensitivity. Blood Pressure, 2005. 14(3): p. 159-169.
[17] Ritvanen, T., et al., Responses of the autonomic nervous system during periods of perceived high and low work stress in younger and older female teachers. Applied Ergonomics, 2006. 37(3): p. 311-318.
[18] Shusterman, V. and O. Barnea, Sympathetic nervous system activity in stress and biofeedback relaxation. IEEE Engineering in Medicine and Biology Magazine, 2005. 24(2): p. 52-57.
[19] Steptoe, A. and Y. Sawada, Assessment of Baroreceptor Reflex Function During Mental Stress and Relaxation. Psychophysiology, 1989. 26(2): p. 140-147.
[20] Akselrod S, Gordon D, Ubel FA, Shannon DC, Barger AC,Cohen RJ (1981) Power spectrum analysis of heart rate fluctuation:a quantitative probe of beat-to-beat cardiovascular control. Science 213:220-222
[21] Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology, “Heart rate variability, Standards of measurement, physiological interpretation and clinical use＂, European Heart Journal, 17：pp.354-381, 1996.
[22] V. Shusterman, O. Barnea, Sympathetic nervous system activity in stress and biofeedback relaxation, IEEE Engineering in Medicine and Biology Magazine,pp. 52-57, March/April, 2005.
[23] B.W. Hyndman and J. R. Gregory, "Spectral Analysis of Sinus Arrhythmia During Mental Loading," Ergonomics, vol. 18, pp. 255-270, 1975.
[24] Pagani M, Lombardi F, Guzzetti S, Rimoldi O, Furlan R, Pizzinelli P, Sandrone G, Malfatto G, Dell'Orto S, Piccaluga E, Turiel M, Baselli G, Cerutti S, Malliani A (1986) Power spectral analysis of heart rate and arterial pressure variabilities as a marker of sympatho-vagal interaction in man and in conscious dog. Circ Res 59:178-193.
[25] Luttmann, A., et al., Joint analysis of spectrum and amplitude (JASA) of electrogram applied for the indication of muscular fatigue among surgeons in urology., in Advanced in Occupation Ergonomic and saefty., A. Mital, H. Krueger, and J.E. Menozzi Fernandez, Editors. 1996, Int. Soc. for Occupational Ergonomics and Safety: Cincinnati. p. 523 - 528.
[26] R. Cooper, et al, EEG Technology, Butterworth, 3rd Edition, pp. 1-2, 1980.
[27] 胡慕美，“Ganong 生理學”，合計圖書出版社，200-204 頁，民國八十年。
[28] 洪祖培、蘇明勳、陳榮基: 腦波機操作之實驗 ，中華民國神經學會印，民國74年出版
[29] Nunez, Paul L.；Srinivasan, Ramesh；Westdorp,Andrew F.；Wijesinghe,Ranjith S.；Tucker,Don M.；Silberstein,Richard B.&Cadusch,Peter J.EEG coherency I:statistics,reference electrode,volume conductin,Laplacians,cortical maging,and interpretation at multiple scales,Electroencephalography and Clinical Neurophysiology,Vol:103,Issue:5,PP.499-515,November,1997.
[30] Nunez, Paul L.；Silberstein,Richard B.；Shi,Zhiping；Carpenter,Matthew R.；Srinivasan,Ramesh；Tucker,Don M.；Doran,Scott M.；Cadusch,Peter J.& Wijesinghe,Ranjith S.EEG coherency II:experimental comparisons of multiple measures,Clinical Neurophysiology,Vol:110,Issue:3,PP.469-486,1999.