轉譯調控在蛋白質合成中扮演著很重要的角色，蛋白質合成失調會使細胞產生異常的生理作用，更嚴重的會導致疾病的產生、癌細胞的惡化等情況產生。目前大多採用核糖體結合位置分析來研究轉譯調控機制，藉由核糖體結合位置分析來探討蛋白質合成量的多寡、基因表現量與各個區段核糖體結合的情況。近年來，研究學者紛紛投入全基因組信息的研究當中，促使著核醣體位置分析的研究數據不斷的產出，如何規劃與整合相關數據成了未來的一大需求。目前，有整合RPF(ribosome profiling)數據資料的網站有GWIPS、RPFdb兩大網站，RPFdb、GWIPS網站都有利用圖形化介面呈現核糖體各個位置的結合狀況，而RPFdb網站透過RPKM定義基因表現量，這是在GWIPS網站看不到的。此外，兩個網站有一個共通點，那就是資料進行映射(mapping)是映射到整個全基因組，讓使用者可以觀看整個基因的表現過程，但如果一個基因有多個Isoform，我只能看到基因整體的表現，但是無法去細看各個Isoform的表現差異。為了讓使用者可以深入了解基因底下各個Isoform的表現情況，我們映射的方式改為映射到轉錄組(Transcriptome)上，並且搭配生物分析軟體RSEM來進行映射的分析，然後再經過Normalize每份RPF數據資料，讓基因可以在不同RPF數據資料之間能在同一水平下互相比較基因表現量、Isoform表現量。最後，將RPF數據資料存在資料庫內。使用者只要透過網頁，就能觀看蛋白質合成情形、基因的表現量、Isoform的表現量。我們將此網站命名為RFviewer(ribosome footprinting viewer)，RFviewer網站提供三個功能:(1)利用圖形化介面呈現核糖體各個位置的結合狀況(2)提供基因表現量(3) 提供Isoform表現量。透過RFviewer網站，我們拿了兩種例子來做分析:(1)同一基因底下各個Isoform的表現差異，分別使用了基EGFR、BRCA1、CDH13三種基因(2)基因與基因之間的表現差異，使用了CCNG1、CCNG2兩個基因來互相比較。由此看出RFviewer網站是一個非常強大的網站工具，用來輔助研究學者進行ribosome profiling的研究。RFviewer網址為: http://cosbi4.ee.ncku.edu.tw/RFviewer/

Translational regulation plays an important role in protein synthesis. Misregulation of protein synthesis will induce the abnormal cell physiology and lead to several diseases like inflammation and cancer development. Recently, an emerging technique named "ribosome profiling" was developed to study the translational regulation mechanism. By ribosome binding position analysis to compare the binding pattern of different isoform expression to gain more accurate understanding of protein synthesis in the cell. In order to let the user understand the expression of the gene and isoform, we mapped to the transcriptome. With the RSEM software for mapping analysis, and then through the Normalize each RPF data. So that users can also compare the binding pattern of different isoform expression to gain more accurate understanding of protein synthesis in the cell. Finally, we save the ribosome profiling data into the database. We named the website is RFviewer(ribosome footprinting viewer).The functions of provided are as follows:(1) A viewer which provides ribosome binding positions on mRNAs(2)Gene expression(3)Isoform expression. For ribosome binding position analysis of scholars, RFviewer website will be a very powerful auxiliary tool. RFviewer website is available online at http://cosbi4.ee.ncku.edu.tw/RFviewer/.

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