此篇論文主要利用流式細胞儀分析細胞凋亡型態，並深入探討鈣離子濃度對於細胞內的影響。本實驗使用7.83Hz掃頻區間，磁場強度1mT，暴露時間採取48小時，且在暴露時間內開關切換時間長度為6小時。在此實驗條件下，我們發現到低頻電磁場對於癌細胞影響力甚大，利用MTT assay量測細胞存活率僅30%。我們想了解到低頻電磁場對於細胞整體到底是破壞性或非破壞性。利用流式細胞儀可分析出細胞型態與結構，且透過Annexin V 和 PI染劑可觀察到細胞凋亡特性，在這細胞凋亡情況下又可分為大致兩種(早期凋亡,晚期凋亡)。基本上受EMF暴露後細胞群都落在這兩種區間範圍內。鈣離子在細胞內扮演相當重要的腳色，主要調控各種生物效應，例如細胞增生、細胞壞死、細胞凋亡等等，利用高通量螢光顯微藥物開發系統，可利用光學鏡頭以及螢光雷射量測細胞內鈣離子濃度，分析細胞內鈣離子是否受EMF影響下而上升，使細胞內外離子濃度不均，進而造成凋亡效應。細胞走向凋亡途徑可能造成DNA損傷，使得粒線體膜通透後，形成一連串凋亡途徑。本實驗未經藥物影響可讓細胞走向凋亡途徑，可說是相當成功。

In this paper, flow cytometry was used to analyze the apoptosis pattern. And further study of the effect of calcium ion concentration on the cells. This experiment uses a 7.83 Hz wave frequency range with a magnetic field strength of 1mT and an exposure time of 48 hours. And the switching time is 6 hours during the exposure time. Under the experimental conditions. We found that low frequency electromagnetic fields have a great influence on cancer cells. Cell viability was only 30% using by MTT assay. We want to know that low-frequency electromagnetic fields are destructive or non-destructive to the whole cell. Cell type and structure can be analyzed by flow cytometry. Apoptosis characteristics can be observed by Annexin V and PI. In this case of apoptosis, it can be divided into two types (Early apoptosis, Late apoptosis). Calcium ions play a very important role in the cell, mainly regulating various biological effects, such as cell proliferation, cell necrosis, apoptosis, etc. Using the optical lens & fluorescent laser to measure intracellular calcium ion concentration, analyze whether intracellular calcium ions are affected by EMF. This experiment can make the cells go to the apoptotic pathway without the influence of drugs, which is quite successful.

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