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研究生: 許中駿
Hsu, Chung-Chun
論文名稱: 添加金屬鹽類與生物酵素對拜香燃煙特徵及基因毒性之影響
Addition of metallic salts and an anti-oxidant - their effects on emission characteristics and genotoxicity of burning incense
指導教授: 林達昌
Lin, Ta-Chang
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程學系
Department of Environmental Engineering
論文出版年: 2007
畢業學年度: 95
語文別: 中文
論文頁數: 155
中文關鍵詞: 拜香Ames test重金屬粒徑分布PAHs懸浮微粒
外文關鍵詞: Ames test, suspended particulates, PAHs, size distribution, heavy metal, Incense
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  • 拜香燃煙中含有懸浮微粒以及多環芳香化合物(PAHs),相關研究指出國人鼻咽癌之得患率高,燒香可能為致病因子之ㄧ。本研究期望對拜香燃燒所產生的污染物質有所減量或改善,因此選取市面上國人常用的四種台灣製拜香,分別添加選用的金屬鹽類與生物酵素藥劑,再放入實驗箱內燃燒且以MOUDI收集燃煙中的污染物質,最後以GC/MS分析燃燒拜香所排放的PAHs,以ICP-AES分析拜香添加藥劑後灰中之重金屬含量並推估其排放至空氣中的含量。並以Ames test探討拜香燃煙中粒徑之基因毒性。
    結果顯示不論拜香有無添加藥劑,大致來說在粒徑0.56~1.00 μm之間的懸浮微粒排放係數為最大,而0.32~0.56 μm之間的懸浮微粒排放係數為次大。生物酵素對懸浮微粒減量效果不明顯,添加硝酸鉀和氯化鈉使得拜香在粒徑較大處之凝聚所佔的百分比較大、MMAD值增加。
    在S-PAHs方面,添加生物酵素有助於在粒徑0.56~1.00 μm處大幅度減量而使得total S-PAHs減量許多,但添加硝酸鉀和氯化鈉並無達到期望在最小粒徑減量之功效。在拜香燃燒後金屬排放至空氣中的推估中,含硝酸鉀的拜香其鉀排放至空氣中的量佔香中鉀的百分比為17%~23%,而含氯化鈉的拜香其鈉排放至空氣中的量佔香中鈉的百分比約為57%~67%。最後以添加藥劑之拜香及原始拜香在粒徑0.56~1.00 μm收集到的樣品做Ames test,結果得知四種香中大致來說以添加氯化鈉所造成的致突變菌落數較多,此似乎表示氯化鈉之添加會導致拜香燃燒時致突性產物之增加,故值得進一步的研究。

    Suspended particulates and PAHs are found in incense smoke. This research studied the effect of additives on incense emissions. The motivation of this research was to achieve some reduction of pollutants from burning incense.
    Four types of incense sticks representing different ingredients were chosen for this study. Metallic salts and an anti-oxidant enzyme were added to several types of incense, which were burned in a custom-designed combustion chamber. The combustion products from incenses were collected and analyzed by a GC/MS for PAHs in both particle and gases phases. Alternatively, a MOUDI connected to the chamber was used for particle size distribution study. Finally, both metals in the emitted particles and in the ash were analyzed with an ICP-AES and the emission rates of metals from incenses calculated. The Ames test was used to assess the genotoxicity of particles emitted from burning incense.
    The results showed that regardless of the addition of metallic salts or the anti-oxidant, particles in the range 0.56~1.00 μm and 0.32~0.56 μm respectively accounted for the major and the next contribution in terms of the particle emission factor. Addition of the anti-oxidant did not result in any significant reduction of suspended particulates. However, addition of metallic salts produced higher percentage of condensation in larger particulate size and with an increase in the MMAD.
    As to the particle-phase PAHs, addition of an anti-oxidant would result in significant reduction of particulate in the range of 0.56~1.00 μm, hence also a significant reduction in total S-PAHs. Nevertheless, addition of KNO3 or NaCl did not bring about a reduction of S-PAHs in the finest particle range.
    It was found that 17%~23% of potassium, which was added as KNO3, emitted into the air, while 57%~67% of sodium, which came from NaCl added into the incense, was discharged into the air.
    Finally, Ames test showed that, for all four types of incenses, addition of NaCl yieled much more colonies for particls in the 0.56~1.00 μm range, implying possible generation of more mutagenic pollutants with addition of NaCl in the incense.

    第一章 前言 1 1-1研究動機 1 1-2研究目的 2 第二章 文獻回顧 3 2-1 拜香的介紹 3 2-1.1 拜香的種類及組成 3 2-1.2 拜香燃煙特性 4 2-1.3 拜香燃燒生成物對人體之影響 7 2-2 PAHs之重要性 9 2-2.1 PAHs的定義 9 2-2.2 多環芳香烴化合物(PAHs)之形成機制 14 2-2.3 PAHs毒性評估指標BaPeq 17 2-3 懸浮微粒 18 2-3.1 懸浮微粒形成機制 18 2-3.2 拜香燃煙懸浮微粒的相關研究 19 2-4 拜香原料及其燃燒生成物的金屬成分 20 2-5 安姆氏試驗 22 2-5.1 Ames test原理 22 2-5.2 Ames test之優點 25 第三章 實驗方法與步驟 26 3-1 實驗設計與流程 26 3-2 採樣設計與規劃 28 3-2.1 採樣設備 30 3-3 採樣前之準備 32 3-3.1 添加藥劑之配製 32 3-3.2 樣品之製備與調理 32 3-3.3 濾紙前處理 33 3-3.4 流量校正 33 3-4 樣品分析 35 3-4.1 萃取 35 3-4.2 濃縮 35 3-4.3 淨化 36 3-4.4 再濃縮 36 3-4.5 氣相層析質譜儀(GC/MS)分析 37 3-4.6 灰之重金屬分析 37 3-4.7 微波消化 38 3-4.8 感應耦合電漿原子發射光譜儀(ICP-AES)分析 40 3-5 安姆氏生物毒性測試 40 3-5.1 試驗流程 40 3-5.2 基本需求之設備 41 3-5.3 試驗之配藥與菌種培養 42 3-5.4 S9混合液之製備 45 3-5.5 試驗程序 46 3-5.6 試驗之數據與毒性判定 50 第四章 數據分析之品質保證及品質控制 51 4-1 PAHs 51 4-1.1 PAHs空白試驗 51 4-1.2 PAHs標準品回收率測試 53 4-1.3 PAHs標準品檢量線 53 4-1.4 PAHs重複分析 54 4-2 毒性試驗 58 4-3 重金屬 59 4-3.1 重金屬空白試驗 59 4-3.2 重金屬標準品回收率測試 60 4-3.3 重金屬標準品檢量線 61 4-3.4 重金屬重複分析 61 第五章 結果與討論 63 5-1 添加不同藥劑對拜香燃燒排放污染物特徵的影響 63 5-1.1 拜香之燃燒時間 63 5-1.2 拜香之燃燒速率 64 5-1.3 不同粒徑之懸浮微粒排放係數 65 5-1.4 拜香灰排放係數 76 5-1.5 不同粒徑之S-PAHs濃度及分布 78 5-2 探討添加藥劑之拜香燃燒特徵的相關性 86 5-2.1 添加藥劑對拜香排放之懸浮微粒與燃燒時間之關係 86 5-2.2 添加藥劑對拜香排放之懸浮微粒與S-PAHs之關係 87 5-3 添加藥劑拜香不同粒徑之BaPeq 88 5-4 探討拜香原料金屬成分與其金屬排放量之關係 94 5-4.1 灰中金屬含量 96 5-4.2 拜香香支中金屬含量 98 5-4.3 金屬排放於空氣中的量之推估 100 5-5 粒徑毒性測試(Ames test) 105 第六章 結論與建議 107 6-1 結論 107 6-2 建議 111 參考文獻 112 附錄A 環保署研擬中之室內空氣品質標準草案 127 附錄B 16種PAHs之檢量線(GC/MS) 131 附錄C 3種重金屬之檢量線(ICP-AES) 147 附錄D 3種重金屬之方法偵測極限 149 附錄E 十六種PAHs之離子選擇 154

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