軟骨組織結構由於缺乏了血管分佈，在加上軟骨細胞數目上的限制，使得在生物體在軟骨組織修復更為困難。目前最常見的方法為細胞移植，然而移植所需的軟骨細胞數量需依賴體外的支架培養過程，因此，目前有部分的學者們正著力於研究可在體外成功培養軟骨細胞的支架環境。
本實驗透由模擬人體透明軟骨的生長環境，成功地合成立體支架所需的聚合微粒。於形成聚合微粒的過程中，添加膠聯劑1– ethyl-3-(3-dimethylaminopropyl) carbodiimide的作用以增加微粒的穩定性以降低在生物體內的降解速率。本實驗以萃取自台灣鯛魚的膠原蛋白與明膠來代替透明軟骨中的膠原蛋白成份，將所得的膠原蛋白 (Collagen) 以及明膠 (Gelatin) 分別與玻尿酸 (Hyaluronic acid)、軟骨硫素(chondroitin – 6-sulfate)膠聯，形成聚合支架，並將其聚合支架研磨成微粒，以微差掃瞄式卡計 (DSC)、紅外線光譜儀 (IR)、掃描式電子顯微鏡 (SEM) 等儀器來鑑定微粒的性質。

Owing to lacking the distribution of vessels in the cartilage and few chondrocyte in human it is more difficult to repair cartilage than other tissue for living organisms. Presently, allograft transplantation is a type of common surgery to repair cartilage. However, the amount of chondrocyte would depend on the three-dimension scaffold which would offer favorable conditions for chondrocyte growth in vitro. Therefore, several researchers investigated the creation of three-dimension scaffold to culture the chondrocyte.
The micro-particles constituting three-dimension scaffold were successfully synthesized in vitro by simulating the beneficial and natural conditions for hyaline cartilage growth. During the synthesis of micro-particles, the stability of micro-particles was increased by adding the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide cross linking agents for decreasing the degrading rate in vivo. In this study, the collagen and gelatin extracted from Taiwan Tilapia were substituted for the major components of human hyaline cartilage. Additionally, the poly micro-particle scaffold would be constructed as a result of the collagen and gelatin interacting with hyaluronic acid and chondroitin-6-sulfate. Finally, the poly micro-particle scaffold would be grinded into the micro-particles and micro-particles would be analyzed by differential scanning calorimetry, IR, and scanning electron microscope.

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