本論文主要利用雙光子激發(two-photon excited，TPE)產生光化學(photochemistry)之技術製作具金奈米柱(gold nanorods，AuNRs)三維微米結構與元件，藉由飛秒(femtosecond，fs)雷射的雙光子吸收而激發光起始劑孟加拉玫瑰素(Rose Bengal，RB)與共同起始劑三乙醇胺(TEA)進行電子轉移因而誘發高分子反應單體如TMPTA或Acrylamide進行連鎖雙光子聚合反應(two-photon polymerization，TPP)。然而，本文將AuNRs事先均勻融入聚合反應的溶液中，於聚合反應的發生同時將金奈米柱固化於結構中，進而製作一個3D微米結構使其具有表面電漿子(surface plasmons，SPs)之特性，並且本文中模擬SPs激發條件與AuNRs於近紅外光的一些光電特性。
TPE光化學技術製作時AuNRs會吸收飛秒雷射瞬間能量，造成SPs振動而伴隨大量熱能產生，瞬間高溫將超過金的熔點，使其AuNRs產生光熱形變(photothermal reshaping)成為圓球狀，而造成此AuNRs失去原本於近紅外光的光電特性。經理論模擬與參考文獻，聚合反應所使用的具有100 fs脈衝寬度之飛秒雷射能量必須低於0.5 mW，因此本論文進行不同波長下雙光子吸收(two-photon absorption，TPA)情形之討論。實驗結果可知針對本文所使用的光起始劑RB而言，TPA最大值在雷射波長為715 nm，因此使用此激發波長來降低TPP反應時所使用的雷射能量瓦特數，使其低於AuNR造成光熱變形的飛秒雷射能量閥值。完成之三維微奈米電漿子結構由掃描式電子顯微鏡(scanning electron microscope，SEM)進行確認。最後，將具AuNRs之三維微米結構經低能量的TPE得到雙光子致光(two-photon luminescence，TPL) 影像，同時也可在三維結構裡設計圖樣利用高於AuNR光熱變形之閥值能量，進行光熱變形使其紅外線SPs特性消失，再藉由低能量掃描得到特定圖樣之TPL影像。

In this thesis, a three-dimensional (3D) polyacrylamide microstructure containing gold nanorods (AuNRs) was fabricated successfully by utilizing femtosecond laser-based two-photon polymerization (TPP) with a Rose Bengal (RB) photoinitiator, and can provide a great diversity of optical properties. To maintain AuNRs in the 3D polymer microstructures, the fabrication laser power can be significantly reduced to 1.0 mW by tuning the laser wavelength for the two-photon absorption of RB to improve TPP efficiency, but not for the longitudinal plasmon resonance of AuNRs to photothermally damage AuNRs.
After the TPP processing, a higher laser power, greater than the threshold of the AuNR damage at the wavelength for the longitudinal plasmon resonance, is adopted to reshape the AuNRs into gold nanospheres. Then, the existence of the AuNRs in designated positions of the fabricated 3D microstructures can be achieved. The doped AuNRs with two-photon luminescence also act as contrast agent for internal diagnosis of 3D polymer microstructures.

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