目前世界上已發展出許多機台、模型和分析方法，來研究細胞的各種性質，但大部分都還有改進的空間，因此本研究的主題，是希望能利用與以往不同的分析方法，得到細胞內機械性質的縱向分佈。
以往的分析方法，其機械性質都只用一個值或範圍來表示，但細胞絕對不是一種均質性材料，因此這一個值或範圍其實是細胞內所有結構的機械性質合在一起所表現出來的行為。基於此，本研究將細胞分為三層：細胞骨架、細胞質和細胞核三層結構，再經由實驗與理論分析，得到細胞內部各區域的機械性質與其變化情形。
實驗部分是利用原子力顯微鏡掃描細胞的表面形貌後，在不同區域做數次的力-距離曲線，得到細胞負載和卸載時的實驗數據。理論部分則是將實驗數據，分別帶入負載-壓深以及卸載-壓深的關係式，經由兩式解聯立後得到各個區域的機械性質，由此方法計算機械性質，可避免接觸面積的估算，直接得到較快較正確的結果。另一方面，為了得到細胞內部在不同結構下其縱向機械性質的過渡變化，例如從細胞骨架到細胞質或細胞核，或者從細胞骨架到細胞質再到細胞核。這些情況都能夠利用exponential函數加上一個或兩個權重因子來完整的描述其過渡行為。
經由實驗與理論分析後，確實得到了細胞內三層結構的機械性質以及其內部過渡變化，再將本研究得到的楊氏模數，與曾經研究過同種類細胞的文獻比對後，確定本研究結果介於參考文獻的範圍內，證明了本研究的方法是可行的，也具體的說明細胞內不同高度、不同位置其機械性質都有可能是不同的。

There are many models and analytical methods have been developed in the world to study all kinds of property of the cells at present, but they are still have improved space. The theme of this research is using a new analytical method that different from the past to receive the vertical distribution of mechanical properties in cells.
In the past, the mechanical properties were expressed only used a value or a range; however, cells are not homogeneous material exactly. This value or range is actually the mixed result of the mechanical properties of all structure. In this research, the PC12 cell was divided into three layers: cytoskeleton, cytoplasm and nucleus; the mechanical properties and the interaction of every layer within cells were obtained by experiments and analysis.
The experiment part is that using AFM scanned surface of cell and perform several force-curves in different selected zones to obtain the loading and unloading data of cell. The analysis part is by bringing the experimental data into the load-depth and unload-depth equation, respectively. The values of mechanical properties can be solved simultaneously by combination of two equations. This method gives the advantage that the contact area is not necessary to evaluate since the contact area is not used. On the other hand, in order to get the transition of the PC12 mechanical properties in vertical direction under different structures within cell. For example: cytoskeleton → cytoplasm or nucleus, cytoskeleton → cytoplasm → nucleus. These transitions can be simulated completely by using exponential function and one or two weight factors.
After experiments and analysis, the mechanical properties and the inside transition of three layers have been obtained. Comparing our mechanical properties with those reported by the references, not only prove our proposed analysis is reasonable, but also concretely explain that the mechanical properties possibly change in the different depth and position.

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