本研究利用表面壓－每分子佔據面積等溫線法、熱力學分析、鬆弛實驗，分析混合單分子層中分子間交互作用和氣/液界面上混合單分子層的穩定性，以探討添加雙十六碳鏈磷酸鹽（dihexadecyl phosphate, DHDP）及膽固醇對於不同碳鏈數目之離子對雙親分子（ion pair amphiphile, IPA）dihexadecyldimethylammonium-hexadecylsulfate（DHDA-HS）和hexadecyltrimethylammonium-hexadecylsulfate（HTMA-HS）於氣/液界面上所形成之Langmuir混合單分子層行為的影響。
實驗結果顯示，DHDA-HS單分子層與HTMA-HS單分子層皆屬於分子間作用力較強的結晶型單分子層（crystalline monolayer）。對兩種IPA/膽固醇混合單分子層而言，從等溫線結果可知，添加過量的膽固醇於DHDA-HS單分子層中，會使混合單分子層有膽固醇聚集或結晶的現象發生，但在HTMA-HS/膽固醇混合單分子層中未有此現象發生。由熱力學分析可得出Aex及∆Gex均為負值，而當IPA單分子層處於排列鬆散的狀態時，膽固醇的凝縮效應（condensing effect）較明顯，但若IPA單分子層中分子的排列較緊密時，其效應則較不顯著。整體來說，膽固醇的添加有助於增加兩種IPA/膽固醇混合單分子層的分子間交互作用。然而，由單分子層的鬆弛行為發現，適量地添加膽固醇於DHDA-HS/膽固醇混合單分子層中，可使混合單分子層的穩定性些微增加，然而添加膽固醇於HTMA-HS/膽固醇混合單分子層中，並無法有效提升HTMA-HS單分子層於氣/液界面上的穩定性。
在兩種IPA/DHDP混合單分子層中，等溫線顯示DHDA-HS/DHDP混合單分子層未有分子取代與損失或極少分子取代與損失的情形發生，但在HTMA-HS/DHDP混合單分子層中，卻無法排除分子取代與損失的現象，推論增加碳氫鏈的數目可降低分子取代而造成損失的現象發生。熱力學分析可得出Aex及∆Gex均為負值，顯示DHDP具有與膽固醇相似的凝縮效應。由單分子層鬆弛實驗可知，不論DHDP添加量為何，IPA/DHDP混合單分子層皆可以穩定存在於氣/液界面上。
添加DHDP於莫耳比7/3的HTMA-HS/膽固醇混合單分子層中，由等溫線結果可知，DHDP可使混合單分子層中的分子在氣/液界面上互相混合，並抑制膽固醇聚集或結晶現象的發生。熱力學分析顯示，DHDP的添加會擾亂混合單分子層中分子排列的秩序性，降低混合單分子層的分子間交互作用，當DHDA-HS單分子層中分子的排列較緊密時，膽固醇固醇環的立體障礙變得更顯著，因此混合單分子層較不傾向存在於高表面壓下。

This study investigated the effects of added dihexadecyl phosphate (DHDP) and cholesterol on the Langmuir monolayer behavior of ion pair amphiphiles (IPAs) with different alkyl chain lengths, double-chained hexadecyltrimethylammonium-hexadecylsulfate (HTMA-HS) and triple-chained dihexadecyldimethylammonium-hexadecylsulfate(DHDA-HS), by analyzing molecular interaction and mixed monolayer stability with surface pressure-area isotherms, thermodynamic analysis and relaxation curves. The isotherms and thermodynamic analysis indicated that cholesterol and DHDP possessed the condensing effect on the mixed monolayers. In addition, at a surface pressure of 30 mN/m, the stability of the mixed monolayers became lower with the cholesterol addition, but the stability of the mixed monolayers was good with DHDP addition. Moreover, there was an obvious phase transition at high surface pressures in the DHDA-HS/cholesterol mixed monolayers and the first collapse surface pressure equaled to that of cholesterol. It was suggested that the cholesterol aggregation or crystallization occurred. With the addition of DHDP into the mixed DHDA-HS/cholesterol monolayers at molar ratio of 7/3, adding DHDP could inhibit cholesterol aggregation or crystallization. From thermodynamic analysis, it was found that DHDP would disturb the arrangement of molecules in mixed monolayers. Since the steric barrier of cholesterol became obvious at high surface pressures, the mixed monolayers were unfavorable to stay at air/water interface at high surface pressures.

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