神經系統主要由兩大類群的細胞所組成，分別是神經細胞及膠細胞。過去對於神經系統的研究主要著重在探討神經細胞上。近期有相當多研究顯示，膠細胞的存在對於神經系統功能的運作與神經細胞是同等重要的，因此我的論文分為兩個部分來探討果蠅神經膠細胞的發育與功能。ㄧ是探討果蠅幼蟲時期眼盤中周邊神經包裹膠細胞的移動，另一個則是探討血腦屏障缺失對神經系統功能的影響，兩個部分都各自利用了不同的研究方法。前者，透過分子克隆的方式尋找能標示周邊神經包裹膠細胞的標記基因Ntan-1的增強子，使未來能以活組織培養及螢光攝影 (ex vivo culture and live imaging)的方式，觀察發育過程中膠細胞與感光神經元的交互作用。後者，以Moody-Gal4在發育中的神經周圍下部膠細胞內降低Rho1基因表現量的方式來破壞血腦屏障的完整性。我以螢光染劑注射測試證實神經周圍下部膠細胞發育過程中受損的確使血腦屏障功能缺失。接著透過攀爬實驗測試和嗅覺記憶實驗分別測試血腦屏障功能缺失果蠅的運動能力以及氣味記憶，發現血腦屏障缺失會影響果蠅神經系統的功能。

Nervous system is made of two major cell types, neuron and glia. In the past, studies on the nervous system mainly focused on the discussion of neuron. Recently, more studies have shown that the presence of glial cells is as important as neurons for the function of the nervous system. My thesis is divided into two parts to study the development and function of Drosophila glial cells. One is to explore the movement of glial cells in the Drosophila larval eye disc, and the other is to explore the effect of defective blood-brain barrier (BBB) on the function of the nervous system. Both parts use different research methods. The 1st part of my thesis, to label wrapping glia in the larval eye disc, I dissected genomic regions of the wrapping glia marker gene Ntan-1 to identify its enhancer elements. It will facilitate the future study of using ex vivo culture and live imaging to examining the interaction between glial cells and photoreceptor neurons in the larval eye disc. The 2nd part of my thesis, to study the effect of defective BBB on the nervous system, I used moody-Gal4 to knockdown the expression of Rho1 gene in the developing subperineurial glia for interrupting the formation of intact BBB. Fluorescent dye injection in flies of reduced Rho1 in subperineurial glia validated this experimental approach to disrupt BBB. Results of climbing test and olfactory memory test on flies with defective BBB showed that the impairment of BBB has an effect on the function of the Drosophila nervous system.

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