本研究利用自由基聚合法(free radical polymerization)合成出三種含有不同單體比例之螢光高分子poly(NIPAAm-co-NMA-co-RN), PNNRN。

共聚高分子PNNRN分別由Naphthoquinone-Rhodamine based 螢光單體、親水性高分子NIPAAM、可交聯高分子NMA所組成 : NIPAAM鏈段使高分子具有收縮-膨潤的特性；經化學熱交聯後之NMA鏈段使高分子具有溶劑耐受性，不會回溶於溶劑；本研究設計的naphthoquinone-rhodamine based 螢光單體，對Fe3+具有感測性質，結構中的rhodamine 可與 Fe3+螯合而開環，產生rhodamine之吸收峰。能量可透過跨鍵能量轉移(through-bond energy transfer , TBET)的機制，由 naphthoquinone 轉移到rhodamine，產生螢光放射，可視作為一 off-on 之感測器。並透過靜電紡絲技術(electrospinning technique)將高分子加工成奈米纖維，做為固態螢光感測器應用於環境的感測。

螢光高分子PNNRN對Fe3+顯示出良好的選擇性，並在其餘金屬陽離子的干擾下具備優秀的競爭性。由job plot之結果推論PNNRN與Fe3+是以1:2的當量形成錯合物，螢光滴定曲線結果可觀察到高分子溶液態與纖維態在各自區間的感測上表現出高度線性，也具備低偵測極限，可達到1.21 µM，並由pH值測試證實PNNRN能應用於常見之酸鹼環境之中(pH 值6-10)，且具備短時間感測 Fe3+之能力。雖然數據顯示高分子纖維態的感測性相較溶液態顯得略為遜色，然而高分子纖維態為固態介質，具有便於攜帶、操作簡單等優點，具有應用於固態螢光感測器之優秀潛力。

This study is to synthesize three types of fluorescent polymers, poly(NIPAAm-co-NMA-co-RN) (PNNRN) via free radical polymerization with varying monomer ratios. PNNRN is composed of a naphthoquinone-rhodamine based fluorescent monomer, hydrophilic NIPAAM, and cross-linkable NMA. The NIPAAM segments provide the polymer with shrink-swell properties, while the NMA segments, after chemical and thermal cross-linking, confer solvent resistance. The designed fluorescent monomer is sensitive to Fe3+. After chelation with Fe3+, cause the spirolactam ring in rhodamine unit to open and exhibit an absorption peak. Energy transfer from naphthoquinone to rhodamine results in fluorescence emission, functioning as an off-on sensor.

The polymer is processed into nanofibers via electrospinning for solid-state fluorescent sensor applications. PNNRN demonstrates high selectivity and competitiveness in detecting Fe3+ amidst other metal ions. Job plot results indicate to form chelation complex with 1:2 stoichiometry between PNNRN and Fe3+. Fluorescence titration curves show high sensitivity with Fe3+, and a low detection limit of 1.49 µM. The pH tests confirm its effectiveness in common acidic and basic environments (pH 6-10), with rapid Fe3+ sensing capability. Despite the solid-state sensor's slightly lower sensitivity compared to its solution state, it offers advantages such as portability and ease of use, highlighting its potential as a solid state fluorescent sensor.

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