本論文首先以改良式漢默法(modified Hummers method)製備氧化石墨，並利用左旋精胺酸以微波法同時進行還原與表面修飾，藉此獲得分散性佳的還原氧化石墨烯。其次，合成具有近紅外光上轉換螢光顯影特性之NaYF4:Yb,Er奈米粒子，並於表面包覆二氧化矽奈米殼層，再以(3-三乙氧基矽丙基)丁二酸酐修飾於二氧化矽奈米殼層表面使其羧基化，最後共價鍵結於表面修飾左旋精胺酸的還原氧化石墨烯表面，形成兼具近紅外上轉換螢光顯影與光熱治療功能之奈米複合材料。透過穿透式電子顯微鏡(TEM)、原子力顯微鏡(AFM)、X光繞射分析儀(XRD)、傅立葉轉換紅外線光譜儀(FTIR)、拉曼光譜儀(Raman)、化學分析電子光譜儀(ESCA)、紫外光/可見光/近紅外光光譜儀UV/VIS/NIR)與螢光光譜儀分析產物之粒子形態、粒徑、晶體結構及光學等特性，可證實本研究已成功製備出還原氧化石墨烯、NaYF4:Yb,Er奈米粒子及其奈米複合材料。此外，藉由人類子宮頸癌細胞的硏究，可確認本研究所製得之奈米複合材料確實兼具有近紅外光光熱治療和螢光顯影的功能。

In this thesis, graphite oxide was synthesized by modified Hummers method and then reduced and surface modified with L-arginine via the microwave method to yield the well-dispersed reduced graphene oxide at first. Secondly, the NaYF4:Yb, Er nanoparticles with near infrared (NIR) upconversion fluorescence imaging property were synthesized, surface coated with silica nanoshells, and then further modified with 3-(triethoxysilyl)propylsuccinic anhydride to generate carboxylic groups on the surface of silica nanoshells. Finally, they were covalently bound on the arginine-modified reduced graphene oxide to form the nanocomposite combining the functions of NIR upconversion fluorescence imaging and photothermal therapy. By transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FTIR), Raman spectra, electron spectroscopy for chemical analysis (ESCA), UV/VIS/NIR spectrophometer, and fluorescence spectrophotometer, the products’ morphologies, sizes, crystalline structures, and optical properties were characterized. It was found that the formation of reduced graphene oxide, NaYF4:Yb,Er nanoparticles, and their nanocomposite has been achieved successfully. In addition, by using a HeLa cancer cell line, it was demonstrated this nanocomposite indeed possessed both the functions of NIR photothermal therapy and fluorescence imaging.

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