隨著奈米科技的蓬勃發展，奈米粒子被廣泛的使用在人們周遭的生活中，雖然奈米粒子的應用帶來了生活上的便利，但也帶來了傷害身體健康之潛在危險性。由過去的研究指出當人體暴露在富含奈米粒子的環境當中，奈米粒子可藉由人體呼吸過程的途徑，進入到肺泡組織內，形成奈米粒子團且吸附在肺泡細胞上，並造成肺部系統的損傷，對肺泡細胞的生理功能產生影響，例如：細胞增生、分化功能的減弱以及造成細胞凋零的現象等。儘管奈米粒子對細胞毒性已廣泛地被研究，但是造成奈米粒子聚集在肺泡細胞之作用力仍未被完全理解。
本研究以原子力學顯微鏡 (AFM) 作為吸附力量測工具，並選定目前周遭生活中常用的銀和金奈米粒子作為研究之對象，並將未加入分散劑之奈米粒子所形成之粒子團利用矽烷偶聯劑 (3-mercaptopropyltrimethoxy silane) 接枝於原子力學顯微鏡探針針尖上，進一步針對活體肺泡細胞 (human fetal lung cell) 的表面來進行奈米粒子團和細胞之間吸附力量之量測及評估。評估部分包含改變量測位置、由不同尺寸銀、金奈米粒子所形成的奈米粒子團對於吸附力量的影響。結果顯示，無論使用何種探針 (bare tip、MPTMS_tip、Ag_tip、Au_tip) 在量測區域內於肺泡細胞表面之吸附力量測結果，其吸附力量的分佈情況皆並不會因為量測位置的改變而使得量測的結果有顯著差異。當銀、金奈米粒子尺寸增加時，該奈米粒子所形成的奈米粒子團和細胞膜表面所形成的單一接觸面積 (single effective contact area) 隨之增加，進而造成粒子和細胞表面之間的吸附力量變大。此外，在相同的粒子尺寸下，由於銀、金奈米粒子團表面特性以及銀、金奈米粒子本身團聚特性的不同，使得粒子間團聚力量、粒子間距以及團聚的粒子數量之差異，影響整體團聚的形狀，進而造成金奈米粒子團與肺泡細胞表面之間的吸附力增加。

With the growing popularity of nanotechnology, nanoparticles (NPs) generate great benefits as well as new potential risks in our daily life. According to the past investigation, NPs may be inhaled into lung, then aggregate and adsorb on the lung cells and result in lung damage and other toxicity, such as cell functions, proliferation decline, and so on. Despite lots of researches focus on the cytopathogenic effect of inhaled particles, the nature of the interactions between nanoparticles and lung cells remains largely unknown.
In this study gold and silver NPs were chosen because they are two of most common commercial NPs in our daily life. Atomic force microscopy (AFM) was as force apparatus to measure the interaction between the surface of human fetal lung cell (IMR-90) and NP clusters which are formed in normal environment. Through 3-mercaptopropyltrimethoxy silane (MPTMS), NP clusters were chemisorbed on the apex of AFM tip and then the adhesion forces between the tip and the cell were analyzed. With the increasing size of NP, adhesion force raised since the single effective contact area between NP clusters and the lung cell membrane surface would affect the adhesive condition between NP clusters and the surface. Besides, owing to the surface properties and the agglomeration of gold, gold NP clusters has better affinity to cell surface than silver NP clusters, which resulted in the adhesion force between gold NP clusters and the cell is thus larger than that between silver NP clusters and the cell. For all the adhesion force between lung cells and different tips (bare tip, MPTMS_tip, Ag_tip, Au_tip), there are no significant differences for the adhesion force of each sites on cell membrane.

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