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研究生： 梁文紘
Liang, Wun-Hong
論文名稱： 應用噴墨印表機於紙基晶片上之生化檢測
Bio-sample Detection on Paper-based Devices Using Ink-jet Printers
指導教授： 楊瑞珍
Yang, Ruey-Jen
學位類別： 碩士
Master
系所名稱： 工學院 - 工程科學系
Department of Engineering Science
論文出版年： 2014
畢業學年度： 102
語文別： 英文
論文頁數： 49
中文關鍵詞： 紙基晶片 、比色法 、生化檢測 、點照護檢驗 、噴墨印表機
外文關鍵詞： Paper-based chip, Colorimetry, Point of Care, Bio-sample detection, Ink-jet printer
相關次數： 點閱：117  下載：2 
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    • 本研究已成功的改良家用印表機用於噴敷生化試劑於paper-based chip當中，進行paper-based chip的生產，而在一般的生化實驗室都是使用Pipette將所需要的試劑滴入我們的paper-based chips當中，過程中只能透過手動的方式進行單件式的操作，當需要大量的製造裝置時就會顯得相當耗時，如果要購置一台自動化的生產設備，又礙於造價昂貴，基於成本的考量我們透過改良一般廉價的壓電噴頭印表機，使其能噴敷生化試劑於paper-based chips中，達到低成本的批量生產，而使用印表機製作的優點如下: 成本低廉、印表機的操作方便、 製作快速、精準、重現性好、適用於大量生產。
    而在paper-based chip 設計的部分，我們採用2D的設計以簡化製造的程序，並且實際的應用於檢測AST(GOT)、ALT(GPT)值，依照傳統比色法，利用影像設備擷取影像並將顏色的平均強度數位化，再根據統計數據產生標準曲線。由實驗結果顯示，AST在0~105 (U/liter)濃度範圍內，其最佳的觀測時間為4分鐘，根據顏色的變化產生標準曲線呈線性的分佈(R2=0.982)；ALT在0~125 (U/liter)濃度範圍內，其最佳的觀測時間為1分鐘，根據顏色的變化產生標準曲線呈現出線性的分佈(R2=0.989)，此外我們也證明了不同濃度的葡萄糖對於檢測AST與ALT是不會造成影響的。
    最後希望低成本紙基晶片可以簡單的應用於個人健康照護與應用於醫學上快速的診斷，以達到“點照護檢驗＂POC (point of care) 理念的實現。

    This study successfully improved the home printer for the spraying of biochemical reagents in paper-based chip production. General biochemistry laboratories use pipettes to introduce the required reagent into paper-based chips. One-piece operation is carried out manually, which is a time-consuming process if the devices are produced in large quantities. The use of automatic production saves time; however, such equipment comes at a high cost. Thus, in order to achieve low-cost mass production, this study attempts to improve the low-cost piezoelectric jet head printer that sprays biochemical reagents into paper-based chips. The following are the advantages of using the printer: low cost, ease of operation, high speed, precise, reproducible and applicable to large scale production.

    A 2D chip design was employed to simplify the manufacturing process, and the chips were applied to real AST(GOT) and ALT(GPT) testing. The colorimetric method utilizes video equipment to capture images, averages the intensities of the colors and digitizes the information. It then generates a standard curve based on statistical data. The experimental results suggest that the best observation time for AST detection within the 0–105 U/liter concentration range is four min; the linear distribution of the standard curve established based on changes in color is R2 = 0.982. For ALT detection, the optimal observation time within the 0–125 U/liter concentration range is one min, and the linear distribution of the standard curve established based on changes in color is R2 = 0.989. In addition, we demonstrated the detections of AST and ALT were not affected by the impact of glucose concentration
    Finally, wish the low-cost paper-based chips can be easily applied in personal health care and fast medical diagnosis, allowing the realization of the POC (point of care) concept.

    Abstract III 中文摘要 V 誌謝 VI List of Table IX List of Figures X Chapter 1 Introduction 1 1-1 Lab on a chip 1 1-2 Microfluidics paper-based analytical devices (μPADs) 2 1-3 Motivation and objectives 3 1-4 Literature survey 3 Chapter 2 Theory 15 2-1 Capillarity 15 2-2 Contact angle 16 2-3 Washburn Equation 17 2-4 Color Model 19 2-5 Micro Piezo Technology 21 Chapter 3 Materials and Methods 22 3-1 Modifications to ink-jet printer 22 3-2 Printing Fluids 25 3-3 Paper-based fluidic devices design 28 3-4 Fabrication 29 3-5 The deployment of the detection solution 30 3-5-1 Reagent for AST assay: 30 3-5-2 Reagent for ALT assay: 31 3-6 Instrument 32 i. Pipette 32 ii. Digital camera 33 iii. Analysis software 34 Chapter 4 Results and discussion 35 4-1 The optimal print number of times 35 4-2 The optimal reaction time 36 4-3 Calibration curve production 39 4-4 Evaluation of analytes with potential for assay interference 42 4-5 Comparison between the pipette reagent titration and the printer printed reagent 43 Chapter 5 45 5-1 Conclusions and future works 45 References 47

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