本研究利用微機電系統製程技術之灌注成形法以及微壓印技術，完成聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)濃度晶片之製備，以應用於老鼠肌母細胞(C2C12)對血小板衍生生長因子(Platelet-derived growth factors, PDGF)最適濃度之研究。並成功的製備一個微濃度晶片，可同時間檢測六種蛋白質濃度對細胞遷移之效果，蛋白質檢測實驗方法首先是細胞外基質(Extracellular matrix, ECM)最適濃度選取以利後續實驗進行，探討不同體積百分濃度之ECM(100%、80%、50%、20%、10%、5%、2%、0%)選出最適合老鼠肌母細胞生長之濃度，加速細胞生長速度以利實驗觀察，然後在晶片中固定細胞培養區域並利用微壓印技術壓印出不同濃度之PDGF，觀察其細胞遷移效應之影響；另外經實驗驗證在10%的濃度下細胞生長快速且成本最低，而肌母細胞對於PDGF之最適濃度為10~100 μg/mL，當PDGF濃度過高或過低於此最適濃度時生長效果皆沒有比此範圍濃度好。故本研究所建立的濃度晶片系統，不但可解決傳統藥物測試上無法觀測細胞遷移效果且不能於同一實驗區內進行多種濃度測試的問題，在未來將有助於藥物濃度測試及藥物過敏測試等應用領域的發展。

This thesis presents a new microfluidic Polydimethylsiloxane (PDMS) chip with pre-concentration unit for determining the optimal Platelet-derived growth factors (PDGF) concentration in mouse myoblast cells. First, we determined the optimal concentration of Extracellular matrix (ECM) to accelerate the growth of mouse myoblast cells. Then, we fixed the cells in the cultured area and used the micro-imprinting chip to emboss different concentrations of PDGF. When the concentration of PDGF was between 10 μg/mL and 100 μg/mL, the growth effect was optimal. Therefore, the proposed chip not only solves the efficiency problem of conventional drug test devices, but also can text various drug concentrations in the same region on one chip.

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