生醫材料與細胞間的交互作用對於調控細胞的貼附、遷徙、增生與分化等
生理行為，扮演著相當重要的角色。本研究透過將生醫材料與刺激訊號
(stimulatory cues)結合，開發出可調控細胞幾何形態並同時提供電刺激之功能型
奈米複合纖維，作為新一代的組織培養支架。實驗中，以生物相容性高分子
poly--caprolactone (PCL)與導電性高分子polyaniline (PANi)溶液進行摻混，並
利用含有方向性磁場之收集器進行靜電紡織，成功製備出具有高度順向之導電
性(PCL/PANi)奈米複合纖維。實驗結果證實，含有1 wt%、2 wt%及3 wt% PANi
之奈米複合纖維其導電度與纖維直徑分別為16.24  1.05 mS/cm、27.47  4.96
mS/cm、63.57  6.67 mS/cm 與300.2  54.7 nm、296.9  55.6 nm、257.9  54.5 nm。
由DSC、FTIR 與TGA 的分析結果證實，PCL 與PANi 可均勻混合，彼此之間
並無化學鍵結形成，且PANi 在奈米複合纖維中的含量與摻混時相符。利用XPS
更進一步證明了PANi 的確存在於纖維表面，使得纖維具有導電性。機械性質
方面，隨著PANi 含量的上升，奈米纖維的抗張強度與楊氏模數隨之上升，但
延伸率則是隨之下降。此外，將奈米複合纖維以PBS 浸泡1 週、2 週、4 週後，
纖維之表面型態並無明顯之變化，證實纖維之穩定性。體外細胞實驗部分，本

The interaction of biomaterial and cell plays an important role in controlling
cell adhesion, migration, proliferation, differentiation. In this study we developed
composite nanofiber mats which can provide the topographic cue and electrical
stimulation to the cultured cells simultaneously, as a new generation of tissue
regeneration scaffold. The composite nanofibers were fabricated by electrospinning
of poly--caprolactone (PCL)/polyaniline (PANi) blend solution with the
magnetic-field-assisted electrospinning (MFAES) apparatus. The conductivity and
diameter of the obtained nanofibers with 1 wt%, 2 wt%, 3 wt% PANi content were
16.24  1.05 mS/cm, 27.47  4.96 mS/cm, 63.57  6.67 mS/cm and 300.2  54.7
nm, 296.9  55.6 nm, 257.9  54.5 nm respectively. The DSC and FTIR analysis
showed that the blended nanofiber was mixed uniformly and no chemical bonding
was formed after blending. The XPS result showed the PANi existed on the surface
of nanofibers. The PANi content measured by TGA in the fibers was corresponded
with the prepared PCL/PANi solution. In vitro stability study showed the
morphology of PCL/PANi nanofiber did not change in the PBS for 4 weeks. After
seeding C2C12 myoblasts on the fibers, the results of cell viability assay showed
that the composite fibers were cytocompatible. Additionally, the fibers with higher
conductivity supported the adhesion and proliferation of C2C12 cells and enhanced
myotube extension. These results indicate that electrically conductive nanofibers
can modulate the induction of myoblasts into myotube formation without additional
electrical stimulation, suggesting that these fibers may have potential as a temporary
scaffold for skeletal tissue engineering. In the future, the effect of electrical
stimulation for nanofibers on the function of myoblast C2C12 will be further
explored.

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