近來因有機溶劑使用甚為頻繁，使含氯有機物如三氯乙烯（Trichloroethylene，TCE）成為台灣地下水與土壤常見之污染物。三氯乙烯存在於污染場址地下含水層中時，具有比水重、不易溶解、不易移動之特性，是屬於DNAPL（Dense Nonaqueous-Phase Liquids）的一種污染物。因此，有效的整治程序顯的相當重要。其中，利用好氧性現地微生物來降解含水層中三氯乙烯的方式，因其整治速率快、成本經濟低、過程環保且有效，固成為目前地下水生物整治重要研發方向之一。

許多研究指出現地生物復育整治效率常未能如實驗室理想；主要由於現地生物復育之工程瓶頸，如（1）使用垂直井復育有效半徑不大 (僅約5公尺左右)，（2）電子供給者及電子接受者在地下未能與污染物有效接觸，及（3）好氧共代謝微生物阻塞井篩，導致清除費用龐大。本研究針對以上現地生物復育技術之主要工程瓶頸，研究發展創新之並聯式水平多孔管擠注系統，設計並建立現地三氯乙烯好氧生物共代謝反應槽。現地三氯乙烯好氧生物共代謝試驗顯示本論文之創新擠注系統可增加現地生物復育之有效半徑，平均分布電子接受者及電子供給者在地下含水層，解決微生物阻塞，降低整治成本及提高整治效率。

此外，本論文依據並聯式水平多孔管擠注系統之特性，建立一維流況下生物降解（Biotransformation）之數值模式。藉由模式分析，本研究發現現地甲苯分解菌對三氯乙烯具有高共代謝效率，且在總長12公尺皆具生物活性之反應槽的共代謝反應中，三氯乙烯之降解效率只需6公尺便可達90％。相較過去垂直井系統之生物降解，本模式也反應出（1）現地生物復育有效半徑增加，（2）電子接受者及電子供給者平均分布在地下含水層，及（3）解決微生物阻塞之優點。

Trichloroethylene (TCE) was widely used as a solvent in the second 50 years of the 20th century. Because of uniform disposal practices for spent solvents in the past, it has become a major groundwater contaminant in Taiwan. In-situ biological methods offer promise for restoring aquifer contaminated organics, including TCE. Aerobic cometabolism is a powerful biological process that microorganisms growing on primary substrate produce enzyme and fortuitously transform secondary substrate.

The efficiency of in-situ bioremediation is commonly lower than that observed in the laboratory. It is mainly due to the following limitations encountered in the field applications, i.e., (1) the radius of influence of vertical wells is small, (2) the contact of electron acceptors and electron donors with the contaminants is not effective, and (3) clogging of well screens requires huge work-over and remediation costs. Through the pilot test, it was demonstrated that using horizontal porous tubes injection system to inject toluene-vapor could improve above engineering limitations.

Furthermore, this research constructs a system of partial differential equations describes basic processes of microbial growth, utilization of electron donor and acceptor, degradation of contaminant, advective transport, dispersion, and sorption in porous media. Simulations provide good matches to the observed TCE. It was demonstrated that the efficiency of TCE degradation of 0.43 mg/L of influence TCE could reach 90% in 6 meters.

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