| 研究生: |
鄭匡復 Zheng, Kuang-Fu |
|---|---|
| 論文名稱: |
體外震波消脂術之動物實驗 Animal Experiment of Extracorporeal Shock Wave Lipotripsy |
| 指導教授: |
梁勝明
Liang, Shen-Min 溫志湧 Wen, Chih-Yung |
| 學位類別: |
碩士 Master |
| 系所名稱: |
工學院 - 航空太空工程學系 Department of Aeronautics & Astronautics |
| 論文出版年: | 2009 |
| 畢業學年度: | 97 |
| 語文別: | 中文 |
| 論文頁數: | 78 |
| 中文關鍵詞: | 病理檢驗 、體外震波 、能量強度 、消脂術 、動物實驗 |
| 外文關鍵詞: | Animal experiment, Lipotripsy, energy intensity, Extracorporeal Shock Wave, Pathological examination |
| 相關次數: | 點閱:121 下載:4 |
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體外震波消脂術是運用體外震波聚焦在脂肪細胞內,將震波所產生的空蝕現象來破壞脂肪組織。由體內的酵素分解這些被破壞的脂肪細胞,再經由循環系統將這些脂肪代謝出體外或是再吸收利用,而使得我們的聚焦區也就是我們希望消脂的區域內脂肪細胞大量減少達到消脂的目的。
本研究首先使用PVDF壓力探針測量10kV的電壓所擊發的體外震波在水中以及在脂肪中的壓力波型,再利用積分程式計算出能量強度。之後,使用六月齡雄性紐西蘭大白兔和二月齡LY品系的公豬做為本研究的實驗動物,使用電壓10kV的震波聚焦在實驗動物的脂肪區域中。在震波試驗後,觀察實驗動物的生理情形以及副作用,並犧牲實驗動物取下檢體進行病理檢驗、切片染色,並用顯微鏡觀察證明震波對於實驗動物脂肪的破壞情形。在動物實驗中可以證明震波空蝕效應的能量確實可以破壞生物體內的脂肪細胞,但是同時也會造成一些輕微的副作用如輕微的出血。還有發現以10kV的震波操作電壓擊發震波六百次和震波的聚焦區域內0.112 之高能量密度,即可造成實驗動物內脂肪細胞受到傷害,而此結果可做為未來臨床實驗之參考。
Extracorporeal shock wave lipotripsy is a technology that applies extracorporeal shock wave focusing on an adipose tissue to destroy adipose cells by effects of shock-wave compression and micro-jets from cavitation. The damaged adipose cells are decomposed by body’s enzyme. The circulatory system re-digests or metabolizes the damaged adipose cells. As a result, unwanted fat cells disappear by the non-invasive method.
In this study, a PVDF pressure sensor was used to detect the focused shock form and to measure the focused pressure in water and in adipose tissue at an operation voltage of 10kV. The corresponding energy intensity was calculated. Six-month-old male rabbits of New Zealand breed and two-month-old male pigs of LY breed were used for experiment. After shock wave administration on the chosen adipose tissue of experimental animals, we inspected animal’s physiological conditions and side effects by eyes. After examination, the tested animal was sacrificed and was taken for pathological examination and slice staining. Finally we confirmed the shock wave destruction on the sample by a microscope. Though animal experiments, the energy of shock waves needed for destroying adipose cells was determined. During animal experiments, it was found that a little side effect like slight bleeding was inevitable. Moreover, it was found that 600 shock-wave firings with 10kV voltage setting can damage fat cells, resulting in an energy intensity of 0.112 in the focal area. This result can serve as a reference for future clinical experiments.
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