人類表皮調節素(epiregulin)為表皮生長因子家族成員之一，過去其他
的研究者發現其具有抑制A431 等癌症細胞株生長，以及促進人類表皮角
質細胞等正常細胞生長的生物活性，而實驗室先前的研究中發現，大腸
癌患者中有較高表現量的人類表皮調節素，則其存活率較高，為探討人
類表皮調節素是否具有抑制或促進大腸癌細胞之生長，本研究以基因工
程之方式將人類表皮調節素以大腸桿菌進行生產。表現之重組蛋白主要
具有組胺酸標籤、凝血酶切割位、人類表皮調節素編碼區等三個部分，
並以鎳親和力管柱與逆向層析管柱進行純化或分析，所純化分離之六產
物分子量均與預期之重組人類表皮調節素相同，且均形成三對雙硫鍵，
且六產物的α 螺旋與β 摺版之比例均與原生人類表皮調節素相似。在經過氧化再摺疊的過程後，對其主要產物進行雙硫鍵分析，發現其三對雙硫鍵結合之情形與原生人類表皮調節素之雙硫鍵形成均相同，最後以受體結合測試證明該產物具有與其受體EGFR 結合之能力，且其結合能力
在移除其組胺酸標籤後有增強的現象。本研究成功以大腸桿菌生產出重
組人類表皮調節素，其二級結構之比例與原生人類表皮調節素相似，而
雙硫鍵形成位置則與之完全相同，並具有與其受體EGFR 結合之活性。

In previous study, we know that patients suffer from colon cancer have higher survival rate when they have higher epiregulin mRNA expression level. To study the potential of epiregulin to be a novel protein drug, recombinant human epireguiln is produced by E.coli expression system. There are at least six conformational isomer found in HPLC analysis. Oxidative refolding method was introduced to get more stable conformational isomer. According the result of LC-MS-MS, the disulfide bond linkages of the stable conformational isomer are the same as native epiregulin. Finally, receptor binding assay
was also used to judge the binding ability of recombinant human epireguiln. In conclusion, purified recombinant human epireguiln has correct disulfide bond linkages and the ability to bind its receptor.

施慶彬，基因重組骨粘連蛋白對臍帶間質幹細胞、牙隨幹細胞與脂肪幹
細胞於體外增生影響，國立成功大學生物科技研究所碩士論文，2010。
黃永敬，利用即時聚合酶連鎖反應技術分析人類大腸直腸癌組織中表皮
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