本研究主要探討當藍綠菌細胞受到臭氧破壞後，細胞破損的程度與其胞內代謝物釋出之相關性，實驗以臭氧做為氧化劑，並以自來水水源中兩種常見產臭之藍綠菌做為研究對象，包括浮絲藻(Planktothricoides raciborskii)及微囊藻(Microcystis aeruginosa)，所使用之浮絲藻具有產高濃度2-MIB的能力，而微囊藻會產生β-cyclocitral。實驗前將此兩種藍綠菌培養於經數道過濾之金門太湖原水與BG-11培養基混合之合成水。
　　研究中藍綠菌細胞被臭氧所破壞的程度，以細胞完整性來做表示，並應用螢光染劑FDA(fluorescein diacetate)搭配兩種偵測儀器-流式細胞儀(flow cytometer, FCM)及螢光顯微鏡(epifluorescence microscope, EFM)，測試其完整程度，並應用掃描式電子顯微鏡(scanning electron microscope, SEM)對細胞進行高放大倍率的觀察，以協助判定細胞表面完整情形。在代謝物分析部分，研究中以2-MIB、β-cyclocitral分別做為浮絲藻與微囊藻代謝物的代表，並應用固相微萃取法(solid phase micro-extraction, SPME)搭配氣相層析質譜儀(gas chromatograph/mass spectrometry detector, GC/MSD)對此兩種臭味物質進行分析。
　　研究結果發現臭氧對微囊藻有很好的破壞效果，而浮絲藻對於臭氧則有較強的抗氧化力；同樣以1 mg/L的水中臭氧氧化700,000 cells/mL的浮絲藻與微囊藻，經過30分鐘的氧化結果顯示，浮絲藻的細胞完整性高出微囊藻62%，且其代謝物2-MIB比β-cyclocitral難被臭氧氧化，經氧化後總量β-cyclocitral的濃度減少了84%，但總量2-MIB濃度只減少26%。在其他細胞數及臭氧濃度下亦發現相似之趨勢。
　　以SEM對經臭氧氧化後的兩種藍綠菌進行觀察發現，細胞表面皆會破洞變形且有胞內物外流的現象，但不同點在於微囊藻細胞會扭曲萎縮，但浮絲藻則是細胞結構變得鬆散而有膨脹的情形。

　　The effect of ozonation on the cell integrity and metabolite release for two nauseous cyanobacteria, Microcystis aeruginosa and Planktothricoides raciborskii, is investigated. The two cyanobacteria were grown in the laboratory with filtrated water from Tai-Lake, Kinmen, and with addition of BG11 algae growth medium. A fluorescence technique, combining fluorescein diacetate (FDA) and SYTOX Green with either flow cytometer (FCM) and/or epifluorescence microscope (EFM), was successfully developed for the determination of cell integrity. A solid-phase microextraction (SPME) concentration followed by a gas chromatograph (GC) and mass spectrometric detector (MSD) was employed to measure a metabolite for M. aeruginosa, β-cyclocitral, and 2-MIB for P. raciborskii. A series of oxidation experiments of cyanobacteria-laden water was conducted at different cell concentrations and different ozone dosages. During the experiments, ozone concentration, cell integrity, metabolite concentration, and chlororphyll-a were monitored at different time.
　　Experimental results indicated that M. aeruginosa is very easy to be ruptured by ozone, while P. raciborskii is more resistant to ozone oxidation. At ozone concentration at 1 mg/L and cell concentration at 700,000 cells/mL, 62% cells remained integral for P. raciborskii after 30 min of reaction, while ~100% cells were ruptured for M. aeruginosa. Similar trend of difference for cell integrity for the two strains was also observed for other combinations of ozone and cell concentrations. For the metabolites of the two strains, 2-MIB from P. raciborskii was more difficult to be destructed by ozone, compared to β-cyclocitral from M. aeruginosa. At ozone concentration at 1 mg/L and cell concentration at 700,000 cells/mL, 84% reduction was observed for β-cyclocitral after ozonation for 30 min, and only 26% was for 2-MIB.
　　The microphotos from SEM clearly show that the cell surfaces for both strains were damaged after ozonation, with some substances leaked from the cells. This strengthened the observation based on EFM and ECM that the cells were ruptured after ozonation. From the SEM microphotos, M. aeruginosa cells became twisted and deformed after ozonation, and P. raciborskii cell became loose and inflated.

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