| 研究生: |
游象淳 You, Siang-chun |
|---|---|
| 論文名稱: |
創傷弧菌藍色螢光蛋白在基因重組大腸桿菌生物取像系統應用之研究 The Imaging Analysis of Recombinant Escherichia coli Using The Blue Fluorescence Protein from Vibrio vulnificus |
| 指導教授: |
鄭智元
Cheng, Chu-Yuan |
| 學位類別: |
碩士 Master |
| 系所名稱: |
工學院 - 化學工程學系 Department of Chemical Engineering |
| 論文出版年: | 2008 |
| 畢業學年度: | 96 |
| 語文別: | 中文 |
| 論文頁數: | 68 |
| 中文關鍵詞: | 藍色螢光蛋白 |
| 外文關鍵詞: | Blue Fluorescence Protein |
| 相關次數: | 點閱:102 下載:1 |
| 分享至: |
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海洋創傷弧菌之藍色螢光蛋白與綠色螢光蛋白相同,可當作報導蛋白使用,惟至目前關於藍色螢光蛋白螢光特性研究之報告仍不多見。
本研究主要目的為探討藍色螢光蛋白之螢光蛋白濃度與螢光強度之關聯,並依據此結果將巨大原生質體在螢光顯微鏡下所觀察之螢光強度轉換成所生產之螢光蛋白濃度,以提升巨大原生質體在生物取像系統之泛用性。
Both the blue fluorescence protein (BFP) and green fluorescence protein (GFP) isolated from Vibrio vulnificus could be utilized as reporter proteins. However, unlike GFP, very few studies on BFP have been published to date.
The correlation between the fluorescent intensity and the BFP concentration was examined in this study. After purifying the BFP expressed by Escherichia coli BL21(DE3)/pET-D7, the fluorescence emitted by various concentrations of BFP were correlated. This correlation could transform the emitted fluorescence observed by a fluorescence microscope into BFP concentration, which might enlarge the applicability of E. coli giant protoplasts in a bioimaging system.
參考文獻
Baumann, P., L. Baumann, M. J. Woolkalis, and S. S. Bang,
Evolution relationship in Vibrio and Photobacterium: a basis for a natural classification,” Annu. Rev. Microbial., 37: 369-398 (1983).
Birdsell, D. C., and E. H. Cota-Robles, “Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme Spheroplasts of Escherichia coli,” J. acteriol., 93: 427-437 (1967).
Bullen, J. J., Spalding, P. B., Ward, C. G., and Gutteridge, Hemochromatosis, iron and septicemia caused by Vibrio vulnificus.Arch. Intern.Med.” J. M. 151:1606-1609 (1991)
Campbell, A.K., 'Living light: biochemistry, function and biomedical application,” Essays Biochem., 24: 41-81 (1989).
Chang, C. C., Y. C. Chuang, Y. C. Chen, and M. C. Chang, “Bright fluorescent of a novel protein from Vibrio vulnificus depends on NADPH and the expression of this protein is regulated by a LysR-type regulatory gene,” Biochem. Biophys. Res. Commun., 319: 207-213 (2004).
Chang, C. C., Y. C. Yin, and M. C. Chang, “Fluorescent intensity of a novel NADPH-binding protein of Vibrio vulnificus can be improved by directed evolution,” Biochem. Biophys. Res. Commun., 322: 303-309 (2004).
Costerton, J. W., J. M. Ingram, and K. J. Cheng, “Structure and function of cell envelope of Gram-negative bacteria.”Bacteriolgical Reviews, 87-110 (1974).
Epstein, W., and S. G. Schultz, “Cation transport in Escherichia coli V. Regulation of cation content”, J. Gen. Physiol., 49: 221-234 (1965).
Furtado, Agnelo, and Robert Henry, “Measurement of green
fluorescent protein concentration in single cells by image analysis,”Anal. Biochem., 310: 84-92 (2002).
Hastings, J. W., C. J. Potrikus, S. C. Gupta, M. Kurfurst, and J. C. Makemson, “Biochemistry and physiology of bioluminescent bacteria,” Adv. microb. physiol., 26: 235-291 (1985).
Heeswijk, W. C., Rabenberg, M., Weterhoff, H. V., and Kahn, D. “The genes of the glutamine systhetase adenylation cascade are not regulated by nitrogen in E. coli. Mol. Microbiol. ,” 9:443-457(1993)
Hsu, R. Y., and H. A. Lardy, “Cleland WW. Pigeon liver malic enzyme. V. Kinetic studies,” J. Biol. Chem., 242: 5315-5322 (1967).
Hu, w. and C. C. Cheng, “Expression of aequorea green fluorescent protein in plant cells.” FEBS Letters., 369: 331-334 (1995).
Jornvall, H., B. Persson, M. Krook, S. Atrian, R. Gonzalez-Duarte, J. Jeffery, and D. Ghosh, “Short-chain dehydrogenases/reductases(SDR),” Biochemistry,34: 6003-6013 (1995).
Kahana, J., and P. Silver, “Current protocols in molecular biology,” In Ausabel, F.(ed). Green and Wiley, N.Y. (1996).
Kuroda, T., N. Okuda, N. Saitoh, T. Hiyama, Y. Terasaki, H. Anazawa, A. Hirata, T. Mogi, I. Kusaka, T. Tsuchiya, I. Yabe, "Patch clamp studies on ion pumps of the cytoplasmic membrane of Escherichia coli,” J. Biol. Chem., 273: 16897-16904 (1998).
Kusaka, I., “Growth and division of protoplasts of bacillus megateriumand inhibition of division by pencillin,” J. Bacteriol., 94: 884-887 (1967).
Leive, L., “Studies on the permeability chang produced in coliform bacteria by ethylenediaminetetraacetate,” J. Biol. Chem., 243: 2373-2380 (1968).
Li, B., and S. X. Lin, “Fluorescence-energy transfer in human estradiol 17 beta-dehydrogenase-NADPH complex and studies on the coenzyme binding,” Eur. J. Biochem., 235: 180-186 (1996).
Matz, M. V., A. F. Fradkov, Y. A. Labas, A. P. Savitsky, A. G. Zaraisky, M. L. Markelov, and S. A. Lukyanov, “Fluorescent proteins from nonbioluminescent Anthozoa species,” Nat. Biotechnol., 17: 969-973 (1999).
McDougald D., L. M. Simpson, J. D. Oliver, and M. Hudson,
"Transformation of Vibrio vulnificus by electroporation,” Curren. Microbiol., 28: 289-291 (1994).
Meighen, E. A., “Molecular biology of bacterial bioluminescence,”Microbiol Rev., 55: 123-142 (1991).
Meighen, E. A., and I. Bartlet, “Complementation of subunits from different bacterial luciferases. Evidence for the role of the beta subunit in the bioluminescent mechanism,” J. Biol. Chem., 255: 11181-11187 (1980).
Miksch, G., F. Bettenworth, K. Friehs, and E. Flaschel, “The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli.”Appl. Microbiol. Biotechnol., 69: 312-320 (2005).
Miksch, G., F. Bettenworth, K. Friehs, E. Flaschel, A. Saalbach, and T. W. Nattkemper, “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli.” Appl. Microbiol. Biotechnol., 69: 1-8
(2005).
Morin, J. G., and J. W. Hastings, ”Energy transfer in a bioluminescent system,” J. Cell Physiol., 77: 313-318 (1979).
Morise, H., O. Shimomura, F. H. Johnson, and J. Winant,
"Intermolecular energy transfer in the bioluminescent system ,”Aequorea. Biochemistry., 13: 2656-2662 (1974).
Paparella, M., E. Kolssov, B.K. Fleischmenn, J. Hescheler, and S.Bremer, “The use of quantitative image analysis in the assessment of in vitro embryotoxicity endopoints based on a novel embryonic stem cell clone with endoderm-related GFP expression,” Toxicol. Vitro, 16: 589-597 (2002).
Prakash, Y. S., “Fluorescent and Luminescent Probs,” 2 nd ed., Academic press, 316-330 (1999).
Prescott, Harley, Klein, Microbiology, 5th edition, McGraw Hill, New York, 56-59 (2001).
Schmidt, T. M. , K. Kopecky, and K. H. Nealson, “Bioluminescence of the insect pathogen Xenorhabdus luminescens,” Appl. Environ. Microbiol., 55: 2607-2612 (1989).
Siegele, D. A., L. Campbell, and J. C. Hu, “Green fluorescent protein as a reporter of transcriptional activity I prokaryotic system,” Methods Enzymol., 305: 499-513 (2000).
Su, J.H., Y. C. Chung, Y. C. Tsai, and M. C. Chang, “Cloning and characterization of a blue fluorescent protein from Vibrio vulnificus,” Biochem. Biophys. Res. Commun., 287: 359-365 (2001).
Tacket, C. O., F. Brenner, and P. A. Blake, “Clinical features and an epidemiological study of Vibrio vulnificus infections,” J. Infect. Dis., 149: 558-561 (1984).
Yang, F., L.G. Moss, and G. N. Jr. Phillips, “The molecular structure of green fluorescent protein.” Nat. Biotechnol., 14: 1246-1251 (1996).
Youvan, D.C. and M. E. Michel-Beyerle, “Structure and fluorescence mechanism of GFP,” Nat. Biotechnol., 14: 1219-1220 (1996).
李瑞俞, 以生物取像系統探討在不同培養條件下之基因重組大腸桿
菌內涵體的形成, 成功大學化學工程研究所碩士論文, 1-21
(2007).
林雅婷, 探討創傷弧菌對感測血清、抗菌蛋白、嗜中性球及巨噬
細胞抗性之σ54依賴型雙因子調控系統, 國立成功大學生物
化學暨分子生物學研究所碩士論文, 14-18 (2006)
劉智偉, 創傷弧菌之藍色螢光蛋白應用於革蘭氏陰性菌生物取像系
統之研究, 成功大學化學工程研究所碩士論文, 1-14 (2005).
劉再鈜, 創傷弧菌藍色螢光蛋白應用於革蘭氏陰性菌巨大原生質體
發光特性之研究, 成功大學化學工程研究所碩士論文, 1-18 (2006).